pAAV-hSyn-RFP

價格:1100元

聯系方式:I47-825O-882O

買家導航

載體基本信息

出品公司: Addgene
載體名稱: pAAV-hSyn-RFP
質粒類型: 腺病毒載體;熒光報告載體
高拷貝/低拷貝: 高拷貝
克隆方法: 限制性內切酶,多克隆位點
啟動子: human Synapsin 1
載體大小: 5909 bp
5' 測序引物及序列: --
3' 測序引物及序列: SP6 5'ATTTAGGTGACACTATAG 3'
載體標簽: --
載體抗性: 氨芐青霉素
篩選標記: 紅色熒光蛋白DsRedExpress
克隆菌株: Stbl3 或 NEB Stble
宿主細胞(系): 神經細胞
備注: pAAV-hSyn-RFP載體含有human synapsin 1 啟動子,適合在神經細胞中表達。
產品目錄號: #22907
穩定性: 穩表達
組成型/誘導型: 組成型
病毒/非病毒: 腺病毒

載體質粒圖譜和多克隆位點信息

pAAV-hSyn-RFP載體圖譜



pAAV-hSyn-RFP 多克隆位點

載體簡介

載體序列

LOCUS       pAAV-hSyn-RFP Sequencing Result	5909 bp 	DNA	SYN
DEFINITION  pAAV-hSyn-RFP Sequencing Result
COMMENT     This file is created by Vector NTI
            http://www.vpiqnf.icu/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pAAV-hSyn-RFP|   
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="pAAV-hSyn-RFP Sequencing Result"
                     /mol_type="other DNA"
     misc_feature    845..1228
                     /label="bGlob_int"
     CDS             1154..2008
                     /label="ORF frame 2"
                     /translation="MFIPLIFLPQLLGNVLVCVLAHHFGKAREFEDLAASASRTSGSP
                     GLQEFDATGRHHGLLRGRHQGVHALQGAHGGLRERPRVRDRGRGRGPPLRGHPDRQAE
                     GDQGRPPALRLGHPVPPVPVRLQGVREAPRRHPRLQEAVLPRGLQVGARDELRGRRRG
                     DRDPGLLPAGRLLHLQGEVHRRELPLRRPRNAEEDYGLGGLHRAPVPPRRRAEGRDPQ
                     GPEAEGRRPLPGGVQVHLHGQEARAAARLLLRGLQAGHHLPQRGLHHRGAVRARRGPP
                     PPVPVAAK*"
     misc_feature    1265..1281
                     /label="pBluescriptSK_primer"
                     /translation="MFIPLIFLPQLLGNVLVCVLAHHFGKAREFEDLAASASRTSGSP
                     GLQEFDATGRHHGLLRGRHQGVHALQGAHGGLRERPRVRDRGRGRGPPLRGHPDRQAE
                     GDQGRPPALRLGHPVPPVPVRLQGVREAPRRHPRLQEAVLPRGLQVGARDELRGRRRG
                     DRDPGLLPAGRLLHLQGEVHRRELPLRRPRNAEEDYGLGGLHRAPVPPRRRAEGRDPQ
                     GPEAEGRRPLPGGVQVHLHGQEARAAARLLLRGLQAGHHLPQRGLHHRGAVRARRGPP
                     PPVPVAAK*"
     CDS             1302..1997
                     /label="ORF frame 3"
                     /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                     GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                     QYERAEGRHHLFL*"
     gene            1320..1997
                     /label="DsRed_Express_2"
                     /gene="DsRed_Express_2"
                     /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                     GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                     QYERAEGRHHLFL*"
     gene            1323..1994
                     /label="DsRed_Express"
                     /gene="DsRed_Express"
                     /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                     GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                     QYERAEGRHHLFL*"
     misc_feature    complement(1500..1520)
                     /label="dsRed1_N_primer"
                     /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                     GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                     QYERAEGRHHLFL*"
     misc_feature    1912..1935
                     /label="dsRed1_C_primer"
                     /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                     GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                     QYERAEGRHHLFL*"
     misc_feature    complement(2040..2057)
                     /label="Sp6_primer"
                     /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                     GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                     QYERAEGRHHLFL*"
     promoter        complement(2041..2058)
                     /label="Sp6_promoter"
                     /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                     GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                     QYERAEGRHHLFL*"
     misc_feature    complement(2077..2094)
                     /label="BGH_rev_primer"
                     /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                     GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                     QYERAEGRHHLFL*"
     terminator      2080..2271
                     /label="bGH_PA_terminator"
                     /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                     GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                     QYERAEGRHHLFL*"
     rep_origin      2855..3161
                     /label="f1_origin"
                     /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                     GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                     QYERAEGRHHLFL*"
     misc_feature    3952..3974
                     /label="pGEX_3_primer"
                     /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                     GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                     QYERAEGRHHLFL*"
     promoter        4133..4161
                     /label="AmpR_promoter"
                     /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                     GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                     QYERAEGRHHLFL*"
     gene            4203..5063
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MLPVATMASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRD
                     GVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVE
                     QYERAEGRHHLFL*"
     CDS             4203..5063
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      5218..5837
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC
   61 GAGCGCAGCG AGTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
  121 AGGGGTTCCT TGTAGTTAAT GATTAACCCG CCATGCTACT TATCTACGTA GCCATGCTCT
  181 AGAGGATCCT TCGAAAAGCT TCTGCAGAGG GCCCTGCGTA TGAGTGCAAG TGGGTTTTAG
  241 GACCAGGATG AGGCGGGGTG GGGGTGCCTA CCTGACGACC GACCCCGACC CACTGGACAA
  301 GCACCCAACC CCCATTCCCC AAATTGCGCA TCCCCTATCA GAGAGGGGGA GGGGAAACAG
  361 GATGCGGCGA GGCGCGTGCG CACTGCCAGC TTCAGCACCG CGGACAGTGC CTTCGCCCCC
  421 GCCTGGCGGC GCGCGCCACC GCCGCCTCAG CACTGAAGGC GCGCTGACGT CACTCGCCGG
  481 TCCCCCGCAA ACTCCCCTTC CCGGCCACCT TGGTCGCGTC CGCGCCGCCG CCGGCCCAGC
  541 CGGACCGCAC CACGCGAGGC GCGAGATAGG GGGGCACGGG CGCGACCATC TGCGCTGCGG
  601 CGCCGGCGAC TCAGCGCTGC CTCAGTCTGC GGTGGGCAGC GGAGGAGTCG TGTCGTGCCT
  661 GAGAGCGCAG TCGAGGCGCG CCGAGCTCGG ATCCTGAGAA CTTCAGGGTG AGTCTATGGG
  721 ACCCTTGATG TTTTCTTTCC CCTTCTTTTC TATGGTTAAG TTCATGTCAT AGGAAGGGGA
  781 GAAGTAACAG GGTACACATA TTGACCAAAT CAGGGTAATT TTGCATTTGT AATTTTAAAA
  841 AATGCTTTCT TCTTTTAATA TACTTTTTTG TTTATCTTAT TTCTAATACT TTCCCTAATC
  901 TCTTTCTTTC AGGGCAATAA TGATACAATG TATCATGCCT CTTTGCACCA TTCTAAAGAA
  961 TAACAGTGAT AATTTCTGGG TTAAGGCAAT AGCAATATTT CTGCATATAA ATATTTCTGC
 1021 ATATAAATTG TAACTGATGT AAGAGGTTTC ATATTGCTAA TAGCAGCTAC AATCCAGCTA
 1081 CCATTCTGCT TTTATTTTAT GGTTGGGATA AGGCTGGATT ATTCTGAGTC CAAGCTAGGC
 1141 CCTTTTGCTA ATCATGTTCA TACCTCTTAT CTTCCTCCCA CAGCTCCTGG GCAACGTGCT
 1201 GGTCTGTGTG CTGGCCCATC ACTTTGGCAA AGCACGTGAA TTCGAAGATC TGGCCGCCTC
 1261 GGCCTCTAGA ACTAGTGGAT CCCCCGGGCT GCAGGAATTC GATGCTACCG GTCGCCACCA
 1321 TGGCCTCCTC CGAGGACGTC ATCAAGGAGT TCATGCGCTT CAAGGTGCGC ATGGAGGGCT
 1381 CCGTGAACGG CCACGAGTTC GAGATCGAGG GCGAGGGCGA GGGCCGCCCC TACGAGGGCA
 1441 CCCAGACCGC CAAGCTGAAG GTGACCAAGG GCGGCCCCCT GCCCTTCGCC TGGGACATCC
 1501 TGTCCCCCCA GTTCCAGTAC GGCTCCAAGG TGTACGTGAA GCACCCCGCC GACATCCCCG
 1561 ACTACAAGAA GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA GCGCGTGATG AACTTCGAGG
 1621 ACGGCGGCGT GGTGACCGTG ACCCAGGACT CCTCCCTGCA GGACGGCTCC TTCATCTACA
 1681 AGGTGAAGTT CATCGGCGTG AACTTCCCCT CCGACGGCCC CGTAATGCAG AAGAAGACTA
 1741 TGGGCTGGGA GGCCTCCACC GAGCGCCTGT ACCCCCGCGA CGGCGTGCTG AAGGGCGAGA
 1801 TCCACAAGGC CCTGAAGCTG AAGGACGGCG GCCACTACCT GGTGGAGTTC AAGTCCATCT
 1861 ACATGGCCAA GAAGCCCGTG CAGCTGCCCG GCTACTACTA CGTGGACTCC AAGCTGGACA
 1921 TCACCTCCCA CAACGAGGAC TACACCATCG TGGAGCAGTA CGAGCGCGCC GAGGGCCGCC
 1981 ACCACCTGTT CCTGTAGCGG CCAAATGAAT TCCCAAACCG TACGGGAAAC GGCCCTATTC
 2041 TATAGTGTCA CCTAAATGCT AGAGCTCGCT GATCAGCCTC GACTGTGCCT TCTAGTTGCC
 2101 AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT GCCACTCCCA
 2161 CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG TGTCATTCTA
 2221 TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC AATAGCGCGG
 2281 CCGCTCTAGA GCATGGCTAC GTAGATAAGT AGCATGGCGG GTTAATCATT AACTACAAGG
 2341 AACCCCTAGT GATGGAGTTG GCCACTCCCT CTCTGCGCGC TCGCTCGCTC ACTGAGGCCG
 2401 GGCGACCAAA GGTCGCCCGA CGCCCGGGCT TTGCCCGGGC GGCCTCAGTG AGCGAGCGAG
 2461 CGCGCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA
 2521 GCCTGAATGG CGAATGGCGA TTCCGTTGCA ATGGCTGGCG GTAATATTGT TCTGGATATT
 2581 ACCAGCAAGG CCGATAGTTT GAGTTCTTCT ACTCAGGCAA GTGATGTTAT TACTAATCAA
 2641 AGAAGTATTG CGACAACGGT TAATTTGCGT GATGGACAGA CTCTTTTACT CGGTGGCCTC
 2701 ACTGATTATA AAAACACTTC TCAGGATTCT GGCGTACCGT TCCTGTCTAA AATCCCTTTA
 2761 ATCGGCCTCC TGTTTAGCTC CCGCTCTGAT TCTAACGAGG AAAGCACGTT ATACGTGCTC
 2821 GTCAAAGCAA CCATAGTACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT
 2881 TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT
 2941 CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC
 3001 TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA
 3061 TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC
 3121 CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT
 3181 CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT
 3241 GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA TTTAAATATT
 3301 TGCTTATACA ATCTTCCTGT TTTTGGGGCT TTTCTGATTA TCAACCGGGG TACATATGAT
 3361 TGACATGCTA GTTTTACGAT TACCGTTCAT CGATTCTCTT GTTTGCTCCA GACTCTCAGG
 3421 CAATGACCTG ATAGCCTTTG TAGAGACCTC TCAAAAATAG CTACCCTCTC CGGCATGAAT
 3481 TTATCAGCTA GAACGGTTGA ATATCATATT GATGGTGATT TGACTGTCTC CGGCCTTTCT
 3541 CACCCGTTTG AATCTTTACC TACACATTAC TCAGGCATTG CATTTAAAAT ATATGAGGGT
 3601 TCTAAAAATT TTTATCCTTG CGTTGAAATA AAGGCTTCTC CCGCAAAAGT ATTACAGGGT
 3661 CATAATGTTT TTGGTACAAC CGATTTAGCT TTATGCTCTG AGGCTTTATT GCTTAATTTT
 3721 GCTAATTCTT TGCCTTGCCT GTATGATTTA TTGGATGTTG GAATCGCCTG ATGCGGTATT
 3781 TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATG GTGCACTCTC AGTACAATCT
 3841 GCTCTGATGC CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT
 3901 GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC Tccgggagct
 3961 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagACGAAAG GGCCTCGTGA
 4021 TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA
 4081 CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA
 4141 TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA
 4201 GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC
 4261 CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG
 4321 CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC
 4381 CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT
 4441 CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT
 4501 TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT
 4561 TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA
 4621 TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC
 4681 TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA
 4741 TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG
 4801 CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC
 4861 GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT
 4921 CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT
 4981 ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG
 5041 CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG
 5101 ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA
 5161 TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA
 5221 TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA
 5281 AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA
 5341 AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT
 5401 TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT
 5461 TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT
 5521 AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT
 5581 TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA
 5641 CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG
 5701 AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC
 5761 GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA
 5821 AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA
 5881 TGTTCTTTCC TGCGTTATCC CCTGATTCT
//

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