pGL4.24[luc2P/minP]

價格:1500元

聯系方式:I47-825O-882O

買家導航

載體基本信息

出品公司: Promega
載體名稱: pGL4.24[luc2P/minP]
質粒類型: 熒光素酶載體
高拷貝/低拷貝: 高拷貝
克隆方法: --
啟動子: --
載體大小: 4411 bp
5' 測序引物及序列: --
3' 測序引物及序列: --
載體標簽: --
載體抗性: 氨芐青霉素
篩選標記: --
克隆菌株: --
宿主細胞(系): --
備注: 用不穩定熒光素酶測定轉錄反應元件活性的最小啟動子載體。
產品目錄號: --
穩定性: --
組成型/誘導型: --
病毒/非病毒: --

載體質粒圖譜和多克隆位點信息

pGL4.24[luc2PminP]載體圖譜

載體簡介

載體序列

LOCUS       pGL4.24[luc2P minP]                4411 bp ds-DNA     circular SYN 29-JUN-2013
DEFINITION  Vector with a minimal promoter for measuring the activity of 
            transcriptional response elements using destabilized luciferase.
ACCESSION   DQ904456
VERSION     .
KEYWORDS    pGL4.24[luc2P minP] 
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Promega
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, June 13, 2020 from SnapGene Viewer 3.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     misc_feature    1..70
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        77..108
                     /note="minP"
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             141..1790
                     /codon_start=1
                     /product="firefly luciferase"
                     /note="luciferase"
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             1794..1913
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /note="hPEST"
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    1965..2086
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2505..3093)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3293..4153)
                     /codon_start=1
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4258..4306
                     /note="synthetic polyadenylation signal"
     misc_feature    4320..4411
                     /note="pause site"
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt agacactaga gggtatataa tggaagctcg acttccagct tggcaatccg
      121 gtactgttgg taaagccacc atggaagatg ccaaaaacat taagaagggc ccagcgccat
      181 tctacccact cgaagacggg accgccggcg agcagctgca caaagccatg aagcgctacg
      241 ccctggtgcc cggcaccatc gcctttaccg acgcacatat cgaggtggac attacctacg
      301 ccgagtactt cgagatgagc gttcggctgg cagaagctat gaagcgctat gggctgaata
      361 caaaccatcg gatcgtggtg tgcagcgaga atagcttgca gttcttcatg cccgtgttgg
      421 gtgccctgtt catcggtgtg gctgtggccc cagctaacga catctacaac gagcgcgagc
      481 tgctgaacag catgggcatc agccagccca ccgtcgtatt cgtgagcaag aaagggctgc
      541 aaaagatcct caacgtgcaa aagaagctac cgatcataca aaagatcatc atcatggata
      601 gcaagaccga ctaccagggc ttccaaagca tgtacacctt cgtgacttcc catttgccac
      661 ccggcttcaa cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa accatcgccc
      721 tgatcatgaa cagtagtggc agtaccggat tgcccaaggg cgtagcccta ccgcaccgca
      781 ccgcttgtgt ccgattcagt catgcccgcg accccatctt cggcaaccag atcatccccg
      841 acaccgctat cctcagcgtg gtgccatttc accacggctt cggcatgttc accacgctgg
      901 gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag gagctattct
      961 tgcgcagctt gcaagactat aagattcaat ctgccctgct ggtgcccaca ctatttagct
     1021 tcttcgctaa gagcactctc atcgacaagt acgacctaag caacttgcac gagatcgcca
     1081 gcggcggggc gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc ttccacctac
     1141 caggcatccg ccagggctac ggcctgacag aaacaaccag cgccattctg atcacccccg
     1201 aaggggacga caagcctggc gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg
     1261 tggacttgga caccggtaag acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg
     1321 gccccatgat catgagcggc tacgttaaca accccgaggc tacaaacgct ctcatcgaca
     1381 aggacggctg gctgcacagc ggcgacatcg cctactggga cgaggacgag cacttcttca
     1441 tcgtggaccg gctgaagagc ctgatcaaat acaagggcta ccaggtagcc ccagccgaac
     1501 tggagagcat cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg
     1561 acgacgatgc cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga
     1621 ccgagaagga gatcgtggac tatgtggcca gccaggttac aaccgccaag aagctgcgcg
     1681 gtggtgttgt gttcgtggac gaggtgccta aaggactgac cggcaagttg gacgcccgca
     1741 agatccgcga gattctcatt aaggccaaga agggcggcaa gatcgccgtg aattctcacg
     1801 gcttccctcc cgaggtggag gagcaggccg ccggcaccct gcccatgagc tgcgcccagg
     1861 agagcggcat ggatagacac cctgctgctt gcgccagcgc caggatcaac gtctaaggcc
     1921 gcgactctag agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga
     1981 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2041 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     2101 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     2161 cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg agagccttca
     2221 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
     2281 tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc
     2341 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     2401 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     2461 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     2521 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     2581 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2641 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2701 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2761 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2821 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     2881 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     2941 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3001 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3061 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3121 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3181 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3241 tatgagtaaa cttggtctga cagcggccgc aaatgctaaa ccactgcagt ggttaccagt
     3301 gcttgatcag tgaggcaccg atctcagcga tctgcctatt tcgttcgtcc atagtggcct
     3361 gactccccgt cgtgtagatc actacgattc gtgagggctt accatcaggc cccagcgcag
     3421 caatgatgcc gcgagagccg cgttcaccgg cccccgattt gtcagcaatg aaccagccag
     3481 cagggagggc cgagcgaaga agtggtcctg ctactttgtc cgcctccatc cagtctatga
     3541 gctgctgtcg tgatgctaga gtaagaagtt cgccagtgag tagtttccga agagttgtgg
     3601 ccattgctac tggcatcgtg gtatcacgct cgtcgttcgg tatggcttcg ttcaactctg
     3661 gttcccagcg gtcaagccgg gtcacatgat cacccatatt atgaagaaat gcagtcagct
     3721 ccttagggcc tccgatcgtt gtcagaagta agttggccgc ggtgttgtcg ctcatggtaa
     3781 tggcagcact acacaattct cttaccgtca tgccatccgt aagatgcttt tccgtgaccg
     3841 gcgagtactc aaccaagtcg ttttgtgagt agtgtatacg gcgaccaagc tgctcttgcc
     3901 cggcgtctat acgggacaac accgcgccac atagcagtac tttgaaagtg ctcatcatcg
     3961 ggaatcgttc ttcggggcgg aaagactcaa ggatcttgcc gctattgaga tccagttcga
     4021 tatagcccac tcttgcaccc agttgatctt cagcatcttt tactttcacc agcgtttcgg
     4081 ggtgtgcaaa aacaggcaag caaaatgccg caaagaaggg aatgagtgcg acacgaaaat
     4141 gttggatgct catactcgtc ctttttcaat attattgaag catttatcag ggttactagt
     4201 acgtctctca aggataagta agtaatatta aggtacggga ggtattggac aggccgcaat
     4261 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta
     4321 acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc
     4381 agtgcaagtg caggtgccag aacatttctc t
//

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