pBABE-GFP

價格:1250元

聯系方式:I47-825O-882O

買家導航

載體基本信息

出品人: Bob Weinberg  
載體名稱: pBABE-GFP
質粒類型: 逆病毒載體
高拷貝/低拷貝: 高拷貝
克隆方法: 限制性內切酶,多克隆位點
啟動子: MSCV
載體大小: 5183 bp
5' 測序引物及序列: pBABE 5' primer 5'CTTTATCCAGCCCTCAC3'
3' 測序引物及序列: pBABE 3' primer 5'ACCCTAACTGACACACATTCC3'
載體標簽:
載體抗性: 氨芐青霉素
篩選標記: hrGFP
克隆菌株: DH5α、HB101等
宿主細胞(系): 常規哺乳動物細胞系
備注:
推薦使用逆病毒包裝載體:pUMVCpCMV-VSV-G
 
產品目錄號: addgene #10668
穩定性: 穩表達
組成型/誘導型: 組成型
病毒/非病毒: 逆轉錄病毒

載體質粒圖譜和多克隆位點信息

pBABE-GFP載體圖譜



pBABE-GFP多克隆位點

載體簡介

載體序列

LOCUS       pBABE GFP Sequencing Result	5183 bp 	DNA	SYN
DEFINITION  pBABE GFP Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="pBABE GFP Sequencing Result"
                     /mol_type="other DNA"
     misc_feature    64..873
                     /label="psi_plus_pack"
     misc_feature    460..867
                     /label="gag"
     misc_feature    803..825
                     /label="MSCV_primer"
     misc_feature    803..825
                     /label="pLXSN_5_primer"
     misc_feature    841..857
                     /label="pBABE_5_primer"
     misc_feature    complement(931..951)
                     /label="pBABE_3_primer"
     misc_feature    complement(937..1313)
                     /label="SV40_enhancer"
     promoter        949..1217
                     /label="SV40_promoter"
     rep_origin      1116..1193
                     /label="SV40_origin"
     misc_feature    1178..1197
                     /label="SV40pro_F_primer"
     misc_feature    complement(1275..1291)
                     /label="pBluescriptKS_primer"
     CDS             1296..2015
                     /label="ORF frame 3"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     misc_feature    2112..2651
                     /label="5_LTR2"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     gene            complement(3710..4570)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     CDS             complement(3710..4570)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(4612..4640)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    4710..5177
                     /label="5_LTR2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    4716..5177
                     /label="3MoMuLV_LTR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 CTCGTCCGGG ATCGGGAGAC CCCTGCCCAG GGACCACCGA CCCACCACCG GGAGGCAAGC
   61 TGGCCAGCAA CTTATCTGTG TCTGTCCGAT TGTCTAGTGT CTATGACTGA TTTTATGCGC
  121 CTGCGTCGGT ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC
  181 GAGTTCTGAA CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTTGG GGGCCGTTTT
  241 TGTGGCCCGA CCTGAGGAAG GGAGTCGATG TGGAATCCGA CCCCGTCAGG ATATGTGGTT
  301 CTGGTAGGAG ACGAGAACCT AAAACAGTTC CCGCCTCCGT CTGAATTTTT GCTTTCGGTT
  361 TGGAACCGAA GCCGCGCGTC TTGTCTGCTG CAGCGCTGCA GCATCGTTCT GTGTTGTCTC
  421 TGTCTGACTG TGTTTCTGTA TTTGTCTGAA AATTAGGGCC AGACTGTTAC CACTCCCTTA
  481 AGTTTGACCT TAGGTCACTG GAAAGATGTC GAGCGGATCG CTCACAACCA GTCGGTAGAT
  541 GTCAAGAAGA GACGTTGGGT TACCTTCTGC TCTGCAGAAT GGCCAACCTT TAACGTCGGA
  601 TGGCCGCGAG ACGGCACCTT TAACCGAGAC CTCATCACCC AGGTTAAGAT CAAGGTCTTT
  661 TCACCTGGCC CGCATGGACA CCCAGACCAG GTCCCCTACA TCGTGACCTG GGAAGCCTTG
  721 GCTTTTGACC CCCCTCCCTG GGTCAAGCCC TTTGTACACC CTAAGCCTCC GCCTCCTCTT
  781 CCTCCATCCG CCCCGTCTCT CCCCCTTGAA CCTCCTCGTT CGACCCCGCC TCGATCCTCC
  841 CTTTATCCAG CCCTCACTCC TTCTCTAGGC GCCGGCCGGA TCCCAGTGTG GTGGTACGTA
  901 GGAATTCGCC AGCACAGTGG TCGACCCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT
  961 CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA
 1021 GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT
 1081 AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT
 1141 CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG
 1201 CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT
 1261 GCAAAAAGCT TATCGATACC GTCGACCTCG AGACCATGGT GAGCAAGCAG ATCCTGAAGA
 1321 ACACCGGCCT GCAGGAGATC ATGAGCTTCA AGGTGAACCT GGAGGGCGTG GTGAACAACC
 1381 ACGTGTTCAC CATGGAGGGC TGCGGCAAGG GCAACATCCT GTTCGGCAAC CAGCTGGTGC
 1441 AGATCCGCGT GACCAAGGGC GCCCCCCTGC CCTTCGCCTT CGACATCCTG AGCCCCGCCT
 1501 TCCAGTACGG CAACCGCACC TTCACCAAGT ACCCCGAGGA CATCAGCGAC TTCTTCATCC
 1561 AGAGCTTCCC CGCCGGCTTC GTGTACGAGC GCACCCTGCG CTACGAGGAC GGCGGCCTGG
 1621 TGGAGATCCG CAGCGACATC AACCTGATCG AGGAGATGTT CGTGTACCGC GTGGAGTACA
 1681 AGGGCCGCAA CTTCCCCAAC GACGGCCCCG TGATGAAGAA GACCATCACC GGCCTGCAGC
 1741 CCAGCTTCGA GGTGGTGTAC ATGAACGACG GCGTGCTGGT GGGCCAGGTG ATCCTGGTGT
 1801 ACCGCCTGAA CAGCGGCAAG TTCTACAGCT GCCACATGCG CACCCTGATG AAGAGCAAGG
 1861 GCGTGGTGAA GGACTTCCCC GAGTACCACT TCATCCAGCA CCGCCTGGAG AAGACCTACG
 1921 TGGAGGACGG CGGCTTCGTG GAGCAGCACG AGACCGCCAT CGCCCAGCTG ACCAGCCTGG
 1981 GCAAGCCCCT GGGCAGCCTG CACGAGTGGG TGTAATAGGG TACCTTCGAT AAAATAAAAG
 2041 ATTTTATTTA GTCTCCAGAA AAAGGGGGGA ATGAAAGACC CCACCTGTAG GTTTGGCAAG
 2101 CTAGCTTAAG TAACGCCATT TTGCAAGGCA TGGAAAAATA CATAACTGAG AATAGAGAAG
 2161 TTCAGATCAA GGTCAGGAAC AGATGGAACA GCTGAATATG GGCCAAACAG GATATCTGTG
 2221 GTAAGCAGTT CCTGCCCCGG CTCAGGGCCA AGAACAGATG GAACAGCTGA ATATGGGCCA
 2281 AACAGGATAT CTGTGGTAAG CAGTTCCTGC CCCGGCTCAG GGCCAAGAAC AGATGGTCCC
 2341 CAGATGCGGT CCAGCCCTCA GCAGTTTCTA GAGAACCATC AGATGTTTCC AGGGTGCCCC
 2401 AAGGACCTGA AATGACCCTG TGCCTTATTT GAACTAACCA ATCAGTTCGC TTCTCGCTTC
 2461 TGTTCGCGCG CTTCTGCTCC CCGAGCTCAA TAAAAGAGCC CACAACCCCT CACTCGGGGC
 2521 GCCAGTCCTC CGATTGACTG AGTCGCCCGG GTACCCGTGT ATCCAATAAA CCCTCTTGCA
 2581 GTTGCATCCG ACTTGTGGTC TCGCTGTTCC TTGGGAGGGT CTCCTCTGAG TGATTGACTA
 2641 CCCGTCAGCG GGGGTCTTTC ACATGCAGCA TGTATCAAAA TTAATTTGGT TTTTTTTCTT
 2701 AAGTATTTAC ATTAAATGGC CATAGTTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG
 2761 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT
 2821 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC
 2881 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA
 2941 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
 3001 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
 3061 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
 3121 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
 3181 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
 3241 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
 3301 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
 3361 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
 3421 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
 3481 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
 3541 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA
 3601 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT
 3661 GCGGCCGCAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT
 3721 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC
 3781 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA
 3841 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG
 3901 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT
 3961 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA
 4021 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT
 4081 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT
 4141 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG
 4201 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG
 4261 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG
 4321 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA
 4381 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT
 4441 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT
 4501 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT
 4561 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA
 4621 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT
 4681 TTCCCCGAAA AGTGCCACCT GCAGCCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC
 4741 AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGAACAG CTGAATATGG GCCAAACAGG
 4801 ATATCTGTGG TAAGCAGTTC CTGCCCCGGC TCAGGGCCAA GAACAGATGG TCCCCAGATG
 4861 CGGTCCAGCC CTCAGCAGTT TCTAGAGAAC CATCAGATGT TTCCAGGGTG CCCCAAGGAC
 4921 CTGAAATGAC CCTGTGCCTT ATTTGAACTA ACCAATCAGT TCGCTTCTCG CTTCTGTTCG
 4981 CGCGCTTCTG CTCCCCGAGC TCAATAAAAG AGCCCACAAC CCCTCACTCG GGGCGCCAGT
 5041 CCTCCGATTG ACTGAGTCGC CCGGGTACCC GTGTATCCAA TAAACCCTCT TGCAGTTGCA
 5101 TCCGACTTGT GGTCTCGCTG TTCCTTGGGA GGGTCTCCTC TGAGTGATTG ACTACCCGTC
 5161 AGCGGGGGTC TTTCATTTGG GGG
//

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