pFastBacHT A

價格:850元

聯系方式:I47-825O-882O

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pFastBacHTA載體基本信息

出品公司: Invitrogen
載體名稱: pFastBacHTA, pFastBac HTA, pFastBacHT A, pFastBac HT A
質粒類型: 昆蟲細胞表達載體;桿狀病毒表達載體
啟動子: Polyhedrin
表達水平:
克隆方法: 多克隆位點,限制性內切酶
載體大小: 4856 bp
5' 測序引物: pFastbac-F: TATTCCGGATTATTCATACC
3' 測序引物: pFastBac-R: ACAAATGTGGTATGGCTGA
載體標簽: N-His tag
載體抗性: Ampicillin 氨芐
篩選標記: Gentamicin 慶大霉素
備注:
含TEV切割位點
產品目錄號: 10584-027
穩定性: 穩表達 Stable
組成型: 組成型 Constitutive
病毒/非病毒: 桿狀病毒

pFastBacHTA載體質粒圖譜和多克隆位點信息

pFastBacHT A 載體圖譜



pFastBacHT A 多克隆位點

pFastBacHT A 載體特征

pFastBacHTA載體簡介

Bac-to-BacHT Vector Kit 設計用于在大腸桿菌 (E. coli) 中生成桿狀病毒載體后,在 Sf9、Sf21 或 High Five 細胞中表達和純化帶組氨酸標簽的重組蛋白。 pFastBacHT 載體具

有以下特點:

 用于表達蛋白質的多角體強啟動子

 用于簡化克隆的三個讀碼框

 用于輕松純化重組融合蛋白的 N-端 6xHis 標簽

 用于在蛋白質純化后去除組氨酸標簽的 TEV 蛋白酶切割位點



引用及參考文獻

DEF6, a Novel Substrate for the Tec Kinase ITK, Contains a Glutamine-rich Aggregation-prone Region and Forms Cytoplasmic Granules that Co-localize with P-bodies.
Hey F, Czyzewicz N, Jones P, Sablitzky F, 
J Biol Chem (2012) 287:31073-31084
Product usage: na

Cytoskeletal changes regulated by the PAK4 serine/threonine kinase are mediated by LIM kinase 1 and cofilin.
Dan C; Kelly A; Bernard O; Minden A; 
J Biol Chem (2001) 276:32115-32136
Product usage: pFASTBAC/HT and pFASTBAC-1 vectors were used to transfect with Sf9 cells with Cellfectin.

Sp3 Represses Gene Expression via the Titration of Promoter-specific Transcription Factors.
Kennett Sarah B; Moorefield K Scott; Horowitz Jonathan M; 
J Biol Chem (2002) 277:9780-9789
Product usage: Baculovirus stocks encoding Sp1, Sp3, M1, and M2 were prepared using the PCR, appropriate primers, and pCMV4-Sp1/flu, pBSK-Sp3/flu, pCR-M1/flu, 

and pCR-M2/flu as substrates. PCR reactions employed pGEX-M2 using Taq polymerase. Amplified cDNAs were subcloned in pFASTBacHTA.

pFastBacHTA載體序列

LOCUS       pFastBacTHT\A           4856 bp    DNA     circular SYN  2-SEP-2003
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.vpiqnf.icu/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..4856
                     /invitrogen="605"
                     /vntifkey="98"
     CDS             589..1449
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=Amp(R)
     promoter        490..588
                     /invitrogen="2330000"
                     /vntifkey="30"
                     /label=bla\promoter
     rep_origin      2..457
                     /invitrogen="2520000"
                     /vntifkey="33"
                     /label=f1\origin
     CDS             complement(2802..3335)
                     /invitrogen="1150000"
                     /vntifkey="4"
                     /label=Gm(R)
     misc_feature    4119..4222
                     /invitrogen="1520000"
                     /vntifkey="21"
                     /label=MCS
     promoter        complement(3524..3551)
                     /invitrogen="2250000"
                     /vntifkey="29"
                     /label=Pc\promoter
     promoter        3904..4032
                     /invitrogen="2260000"
                     /vntifkey="29"
                     /label=PH\promoter
     primer          3920..3937
                     /invitrogen="2060000"
                     /vntifkey="27"
                     /label=Polyhedrin\forward\primer
     rep_origin      1594..2267
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     polyA_signal    4240..4480
                     /invitrogen="1850000"
                     /vntifkey="25"
                     /label=SV40\pA
     CDS             4101..4121
                     /invitrogen="1300000"
                     /vntifkey="4"
                     /label=TEV\recognition\site
     misc_recomb     4499..4693
                     /invitrogen="2750000"
                     /vntifkey="86"
                     /label=Tn7L
     misc_recomb     2512..2735
                     /invitrogen="2760000"
                     /vntifkey="86"
                     /label=Tn7R
     CDS             4062..4079
                     /invitrogen="1015000"
                     /vntifkey="4"
                     /label=6xHis
     CDS             4050..4052
                     /invitrogen="123000000"
                     /vntifkey="4"
                     /label=initiation\ATG
BASE COUNT     1219 a      1197 c      1152 g      1288 t
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct
     2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
     2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
     2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc
     3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
     3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
     3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
     3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
     3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
     3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
     3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
     3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
     3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
     4021 ccatcgggcg cggatctcgg tccgaaacca tgtcgtacta ccatcaccat caccatcacg
     4081 attacgatat cccaacgacc gaaaacctgt attttcaggg cgccatggat ccggaattca
     4141 aaggcctacg tcgacgagct caactagtgc ggccgctttc gaatctagag cctgcagtct
     4201 cgaggcatgc ggtaccaagc ttgtcgagaa gtactagagg atcataatca gccataccac
     4261 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     4321 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     4381 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     4441 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatcactgc ttgagcctag
     4501 gagatccgaa ccagataagt gaaatctagt tccaaactat tttgtcattt ttaattttcg
     4561 tattagctta cgacgctaca cccagttccc atctattttg tcactcttcc ctaaataatc
     4621 cttaaaaact ccatttccac ccctcccagt tcccaactat tttgtccgcc cacagcgggg
     4681 catttttctt cctgttatgt ttttaatcaa acatcctgcc aactccatgt gacaaaccgt
     4741 catcttcggc tactttttct ctgtcacaga atgaaaattt ttctgtcatc tcttcgttat
     4801 taatgtttgt aattgactga atatcaacgc ttatttgcag cctgaatggc gaatgg
//

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