pNL4-3

價格:10000元

聯系方式:I47-825O-882O

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pNL4.3載體HIV-1載體基本信息

出品公司: NIH
載體名稱: pNL4-3、pNL4.3
載體又名: HIV-1 NL4-3 Infectious Molecular Clone (pNL4-3)、 HIV-1 vector pNL4-3、pNL4-3載體、pNL4.3載體
質粒類型: 哺乳動物細胞載體;逆病毒包裝載體
高拷貝/低拷貝: 高拷貝
克隆方法: 限制性內切酶,多克隆位點
啟動子: CMV
載體大小: 14825 bp
5' 測序引物及序列: --
3' 測序引物及序列: --
載體序列: Genbank: AF324493
存儲人: Dr. Malcolm Mar
載體抗性: 氨芐青霉素
篩選標記: 嘌呤霉素(Puromycin)
克隆菌株: DH5α 等
宿主細胞(系): 常規細胞系(293、CV-1、CHO等)
備注:
一個包含有全長基因,復制能力強,能夠感染HIV-1亞型B NL4-3蛋白的分子克隆載體。
這個載體產生的病毒利用CXCR4作為共同受體。

該載體為大小為14825 bp,包括插入片段。
 
這個嵌合載體的來源是HIV-1ny5分離物5’ 從基因組DNA中直接克隆的LAV分離物3’。前病毒NY5(5’SmaI和 3’EcoRI)前病毒3’片段(5’EcoRI 和3’NruI)被鈍端克隆到PvuII位點的pUC18中 刪除多段連接片段后。
產品目錄號: #114
穩定性: 穩表達
組成型/誘導型: 組成型
病毒/非病毒: 非病毒
生物危害性:
參考文獻:
Adachi, A., Gendelman, H. E., Koenig, S., Folks, T., Willey, R., Rabson, A., & Martin, M. A. (1986). Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone. J Virol, 59(2), 284-291. PUBMED
載體說明書:  pNL4-3載體說明書
相關載體: pNL4-3-Luc-R-E-

pNL4.3載體HIV-1載體質粒圖譜和多克隆位點信息

pNL4-3載體圖譜

pNL4.3載體HIV-1載體簡介

pNL4.3載體HIV-1載體序列

LOCUS       pNL4-3          14825 bp    DNA     circular  SYN 25-JUL-2016
DEFINITION  HIV-1 vector pNL4-3, complete sequence.
ACCESSION   AF324493
VERSION     AF324493.2
KEYWORDS    .
SOURCE      HIV-1 vector pNL4-3
  ORGANISM  HIV-1 vector pNL4-3
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 9709)
  AUTHORS   Adachi,A., Gendelman,H.E., Koenig,S., Folks,T., Willey,R.,
            Rabson,A. and Martin,M.A.
  TITLE     Production of acquired immunodeficiency syndrome-associated
            retrovirus in human and nonhuman cells transfected with an
            infectious molecular clone
  JOURNAL   J. Virol. 59 (2), 284-291 (1986)
   PUBMED   3016298
REFERENCE   2  (bases 1 to 14825)
  AUTHORS   Bosche,W.J., Poon,D.T.K., Ott,D.E., Hu,W.-S. and Gorelick,R.J.
  TITLE     Complete Plasmid Sequence of pNL4-3
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 14825)
  AUTHORS   Bosche,W.J., Poon,D.T.K., Ott,D.E., Hu,W.-S. and Gorelick,R.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2000) AIDS Vaccine Program, SAIC Frederick and
            Drug Resistance Program, National Cancer Institute at Frederick, PO
            Box B, Frederick, Maryland 21702-1202, USA
REFERENCE   4  (bases 1 to 14825)
  AUTHORS   Strebel,K.J. and Martin,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2010) NIAID, Viral Biochemistry, National
            Institutes of Health, Bethesda, MD, USA
  REMARK    Sequence update by submitter
COMMENT     On May 24, 2010 this sequence version replaced AF324493.1.
FEATURES             Location/Qualifiers
     source          1..14825
                     /organism="HIV-1 vector pNL4-3"
                     /mol_type="other DNA"
                     /db_xref="taxon:151458"
                     /plasmid="pNL4-3"
                     /focus
     source          1..9709
                     /organism="Human immunodeficiency virus 1"
                     /proviral
                     /mol_type="other DNA"
                     /strain="NY5/BRU (LAV-1)"
                     /db_xref="taxon:11676"
                     /note="from sequence deposited in GenBank Accession Number
                     M19921"
     repeat_region   1..634
                     /note="5' long terminal repeat"
                     /rpt_type=long_terminal_repeat
     repeat_region   454..550
                     /note="R; 5' copy"
     CDS             790..2292
                     /codon_start=1
                     /transl_table=11
                     /product="gag polyprotein"
                     /protein_id="AAK08483.1"
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERF
                     AVNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALD
                     KIEEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVE
                     EKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPV
                     HAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRM
                     YSPTSILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTIL
                     KALGPGATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKC
                     FNCGKEGHIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQ
                     SRPEPTAPPEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             <2085..5096
                     /codon_start=1
                     /transl_table=11
                     /product="pol polyprotein"
                     /protein_id="AAK08484.2"
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEA
                     GADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKM
                     IGGIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPI
                     ETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAI
                     KKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLD
                     KDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIV
                     IYQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPD
                     KWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPL
                     TEEAELELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGK
                     YARMKGAHTNDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWI
                     PEWEFVNTPPLVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPL
                     TDTTNQKTELQAIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIK
                     KEKVYLAWVPAHKGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASD
                     FNLPPVVAKEIVASCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGY
                     IEAEVIPAETGQETAYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGI
                     PYNPQSQGVIESMNKELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGER
                     IVDIIATDIQTKELQKQITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDI
                     KVVPRRKAKIIRDYGKQMAGDDCVASRQDED"
     misc_difference 2421
                     /note="compared to sequence deposited in GenBank Accession
                     Number M19921; results in arginine to glycine
                     substitution"
                     /replace="g"
     CDS             5041..5619
                     /codon_start=1
                     /transl_table=11
                     /product="vif protein"
                     /protein_id="AAK08482.1"
                     /translation="MENRWQVMIVWQVDRMRINTWKRLVKHHMYISRKAKDWFYRHHY
                     ESTNPKISSEVHIPLGDAKLVITTYWGLHTGERDWHLGQGVSIEWRKKRYSTQVDPDL
                     ADQLIHLHYFDCFSESAIRNTILGRIVSPRCEYQAGHNKVGSLQYLALAALIKPKQIK
                     PPLPSVRKLTEDRWNKPQKTKGHRGSHTMNGH"
     CDS             5559..5849
                     /codon_start=1
                     /transl_table=11
                     /product="vpr protein"
                     /protein_id="AAK08485.1"
                     /translation="MEQAPEDQGPQREPYNEWTLELLEELKSEAVRHFPRIWLHNLGQ
                     HIYETYGDTWAGVEAIIRILQQLLFIHFRIGCRHSRIGVTRQRRARNGASRS"
     misc_feature    5743..5748
                     /note="EcoRI site of recombination"
     CDS             join(5830..6044,8369..8414)
                     /codon_start=1
                     /transl_table=11
                     /product="tat protein"
                     /protein_id="AAK08486.1"
                     /translation="MEPVDPRLEPWKHPGSQPKTACTNCYCKKCCFHCQVCFMTKALG
                     ISYGRKKRRQRRRAHQNSQTHQASLSKQPTSQSRGDPTGPKE"
     CDS             join(5969..6044,8369..8643)
                     /codon_start=1
                     /transl_table=11
                     /product="rev protein"
                     /protein_id="AAK08487.1"
                     /translation="MAGRSGDSDEELIRTVRLIKLLYQSNPPPNPEGTRQARRNRRRR
                     WRERQRQIHSISERILSTYLGRSAEPVPLQLPPLERLTLDCNEDCGTSGTQGVGSPQI
                     LVESPTVLESGTKE"
     CDS             6061..6306
                     /codon_start=1
                     /transl_table=11
                     /product="vpu protein"
                     /protein_id="AAK08488.1"
                     /translation="MQPIIVAIVALVVAIIIAIVVWSIVIIEYRKILRQRKIDRLIDR
                     LIERAEDSGNESEGEVSALVEMGVEMGHHAPWDIDDL"
     CDS             6221..8785
                     /codon_start=1
                     /transl_table=11
                     /product="envelope polyprotein"
                     /protein_id="AAK08489.2"
                     /translation="MRVKEKYQHLWRWGWKWGTMLLGILMICSATEKLWVTVYYGVPV
                     WKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLVNVTENFNMWKNDMVE
                     QMHEDIISLWDQSLKPCVKLTPLCVSLKCTDLKNDTNTNSSSGRMIMEKGEIKNCSFN
                     ISTSIRDKVQKEYAFFYKLDIVPIDNTSYRLISCNTSVITQACPKVSFEPIPIHYCAP
                     AGFAILKCNNKTFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEDVVIRSANFT
                     DNAKTIIVQLNTSVEINCTRPNNNTRKSIRIQRGPGRAFVTIGKIGNMRQAHCNISRA
                     KWNATLKQIASKLREQFGNNKTIIFKQSSGGDPEIVTHSFNCGGEFFYCNSTQLFNST
                     WFNSTWSTEGSNNTEGSDTITLPCRIKQFINMWQEVGKAMYAPPISGQIRCSSNITGL
                     LLTRDGGNNNNGSEIFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREK
                     RAVGIGALFLGFLGAAGSTMGAASMTLTVQARQLLSDIVQQQNNLLRAIEAQQHLLQL
                     TVWGIKQLQARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNNM
                     TWMEWDREINNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKL
                     FIMIVGGLVGLRIVFAVLSIVNRVRQGYSPLSFQTHLPIPRGPDRPEGIEEEGGERDR
                     DRSIRLVNGSLALIWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEALKYWWNLL
                     QYWSQELKNSAVNLLNATAIAVAEGTDRVIEVLQAAYRAIRHIPRRIRQGLERILL"
     CDS             8787..9407
                     /codon_start=1
                     /transl_table=11
                     /product="nef protein"
                     /protein_id="AAK08490.1"
                     /translation="MGGKWSKSSVIGWPAVRERMRRAEPAADGVGAVSRDLEKHGAIT
                     SSNTAANNAACAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH
                     SQRRQDILDLWIYHTQGYFPDWQNYTPGPGVRYPLTFGWCYKLVPVEPDKVEEANKGE
                     NTSLLHPVSLHGMDDPEREVLEWRFDSRLAFHHVARELHPEYFKNC"
     repeat_region   9076..9709
                     /note="3' long terminal repeat"
                     /rpt_type=long_terminal_repeat
     repeat_region   9529..9626
                     /note="R; 3' copy"
     regulatory      9602..9607
                     /regulatory_class="polyA_signal_sequence"
     misc_feature    9710..11283
                     /note="3' cellular genomic sequence"
     CDS             complement(12279..13139)
                     /note="from pUC vector"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="AAK08491.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     misc_feature    13648..14825
                     /note="5' cellular genomic sequence"
ORIGIN      
        1 tggaagggct aatttggtcc caaaaaagac aagagatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
      121 tgacctttgg atggtgcttc aagttagtac cagttgaacc agagcaagta gaagaggcca
      181 atgaaggaga gaacaacagc ttgttacacc ctatgagcca gcatgggatg gaggacccgg
      241 agggagaagt attagtgtgg aagtttgaca gcctcctagc atttcgtcac atggcccgag
      301 agctgcatcc ggagtactac aaagactgct gacatcgagc tttctacaag ggactttccg
      361 ctggggactt tccagggagg tgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 gctacatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgctca aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagtaaa gccagaggag atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcggta ttaagcgggg gagaattaga taaatgggaa
      841 aaaattcggt taaggccagg gggaaagaaa caatataaac taaaacatat agtatgggca
      901 agcagggagc tagaacgatt cgcagttaat cctggccttt tagagacatc agaaggctgt
      961 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1021 ttatataata caatagcagt cctctattgt gtgcatcaaa ggatagatgt aaaagacacc
     1081 aaggaagcct tagataagat agaggaagag caaaacaaaa gtaagaaaaa ggcacagcaa
     1141 gcagcagctg acacaggaaa caacagccag gtcagccaaa attaccctat agtgcagaac
     1201 ctccaggggc aaatggtaca tcaggccata tcacctagaa ctttaaatgc atgggtaaaa
     1261 gtagtagaag agaaggcttt cagcccagaa gtaataccca tgttttcagc attatcagaa
     1321 ggagccaccc cacaagattt aaataccatg ctaaacacag tggggggaca tcaagcagcc
     1381 atgcaaatgt taaaagagac catcaatgag gaagctgcag aatgggatag attgcatcca
     1441 gtgcatgcag ggcctattgc accaggccag atgagagaac caaggggaag tgacatagca
     1501 ggaactacta gtacccttca ggaacaaata ggatggatga cacataatcc acctatccca
     1561 gtaggagaaa tctataaaag atggataatc ctgggattaa ataaaatagt aagaatgtat
     1621 agccctacca gcattctgga cataagacaa ggaccaaagg aaccctttag agactatgta
     1681 gaccgattct ataaaactct aagagccgag caagcttcac aagaggtaaa aaattggatg
     1741 acagaaacct tgttggtcca aaatgcgaac ccagattgta agactatttt aaaagcattg
     1801 ggaccaggag cgacactaga agaaatgatg acagcatgtc agggagtggg gggacccggc
     1861 cataaagcaa gagttttggc tgaagcaatg agccaagtaa caaatccagc taccataatg
     1921 atacagaaag gcaattttag gaaccaaaga aagactgtta agtgtttcaa ttgtggcaaa
     1981 gaagggcaca tagccaaaaa ttgcagggcc cctaggaaaa agggctgttg gaaatgtgga
     2041 aaggaaggac accaaatgaa agattgtact gagagacagg ctaatttttt agggaagatc
     2101 tggccttccc acaagggaag gccagggaat tttcttcaga gcagaccaga gccaacagcc
     2161 ccaccagaag agagcttcag gtttggggaa gagacaacaa ctccctctca gaagcaggag
     2221 ccgatagaca aggaactgta tcctttagct tccctcagat cactctttgg cagcgacccc
     2281 tcgtcacaat aaagataggg gggcaattaa aggaagctct attagataca ggagcagatg
     2341 atacagtatt agaagaaatg aatttgccag gaagatggaa accaaaaatg atagggggaa
     2401 ttggaggttt tatcaaagta agacagtatg atcagatact catagaaatc tgcggacata
     2461 aagctatagg tacagtatta gtaggaccta cacctgtcaa cataattgga agaaatctgt
     2521 tgactcagat tggctgcact ttaaattttc ccattagtcc tattgagact gtaccagtaa
     2581 aattaaagcc aggaatggat ggcccaaaag ttaaacaatg gccattgaca gaagaaaaaa
     2641 taaaagcatt agtagaaatt tgtacagaaa tggaaaagga aggaaaaatt tcaaaaattg
     2701 ggcctgaaaa tccatacaat actccagtat ttgccataaa gaaaaaagac agtactaaat
     2761 ggagaaaatt agtagatttc agagaactta ataagagaac tcaagatttc tgggaagttc
     2821 aattaggaat accacatcct gcagggttaa aacagaaaaa atcagtaaca gtactggatg
     2881 tgggcgatgc atatttttca gttcccttag ataaagactt caggaagtat actgcattta
     2941 ccatacctag tataaacaat gagacaccag ggattagata tcagtacaat gtgcttccac
     3001 agggatggaa aggatcacca gcaatattcc agtgtagcat gacaaaaatc ttagagcctt
     3061 ttagaaaaca aaatccagac atagtcatct atcaatacat ggatgatttg tatgtaggat
     3121 ctgacttaga aatagggcag catagaacaa aaatagagga actgagacaa catctgttga
     3181 ggtggggatt taccacacca gacaaaaaac atcagaaaga acctccattc ctttggatgg
     3241 gttatgaact ccatcctgat aaatggacag tacagcctat agtgctgcca gaaaaggaca
     3301 gctggactgt caatgacata cagaaattag tgggaaaatt gaattgggca agtcagattt
     3361 atgcagggat taaagtaagg caattatgta aacttcttag gggaaccaaa gcactaacag
     3421 aagtagtacc actaacagaa gaagcagagc tagaactggc agaaaacagg gagattctaa
     3481 aagaaccggt acatggagtg tattatgacc catcaaaaga cttaatagca gaaatacaga
     3541 agcaggggca aggccaatgg acatatcaaa tttatcaaga gccatttaaa aatctgaaaa
     3601 caggaaagta tgcaagaatg aagggtgccc acactaatga tgtgaaacaa ttaacagagg
     3661 cagtacaaaa aatagccaca gaaagcatag taatatgggg aaagactcct aaatttaaat
     3721 tacccataca aaaggaaaca tgggaagcat ggtggacaga gtattggcaa gccacctgga
     3781 ttcctgagtg ggagtttgtc aatacccctc ccttagtgaa gttatggtac cagttagaga
     3841 aagaacccat aataggagca gaaactttct atgtagatgg ggcagccaat agggaaacta
     3901 aattaggaaa agcaggatat gtaactgaca gaggaagaca aaaagttgtc cccctaacgg
     3961 acacaacaaa tcagaagact gagttacaag caattcatct agctttgcag gattcgggat
     4021 tagaagtaaa catagtgaca gactcacaat atgcattggg aatcattcaa gcacaaccag
     4081 ataagagtga atcagagtta gtcagtcaaa taatagagca gttaataaaa aaggaaaaag
     4141 tctacctggc atgggtacca gcacacaaag gaattggagg aaatgaacaa gtagataaat
     4201 tggtcagtgc tggaatcagg aaagtactat ttttagatgg aatagataag gcccaagaag
     4261 aacatgagaa atatcacagt aattggagag caatggctag tgattttaac ctaccacctg
     4321 tagtagcaaa agaaatagta gccagctgtg ataaatgtca gctaaaaggg gaagccatgc
     4381 atggacaagt agactgtagc ccaggaatat ggcagctaga ttgtacacat ttagaaggaa
     4441 aagttatctt ggtagcagtt catgtagcca gtggatatat agaagcagaa gtaattccag
     4501 cagagacagg gcaagaaaca gcatacttcc tcttaaaatt agcaggaaga tggccagtaa
     4561 aaacagtaca tacagacaat ggcagcaatt tcaccagtac tacagttaag gccgcctgtt
     4621 ggtgggcggg gatcaagcag gaatttggca ttccctacaa tccccaaagt caaggagtaa
     4681 tagaatctat gaataaagaa ttaaagaaaa ttataggaca ggtaagagat caggctgaac
     4741 atcttaagac agcagtacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     4801 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     4861 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     4921 gagatccagt ttggaaagga ccagcaaagc tcctctggaa aggtgaaggg gcagtagtaa
     4981 tacaagataa tagtgacata aaagtagtgc caagaagaaa agcaaagatc atcagggatt
     5041 atggaaaaca gatggcaggt gatgattgtg tggcaagtag acaggatgag gattaacaca
     5101 tggaaaagat tagtaaaaca ccatatgtat atttcaagga aagctaagga ctggttttat
     5161 agacatcact atgaaagtac taatccaaaa ataagttcag aagtacacat cccactaggg
     5221 gatgctaaat tagtaataac aacatattgg ggtctgcata caggagaaag agactggcat
     5281 ttgggtcagg gagtctccat agaatggagg aaaaagagat atagcacaca agtagaccct
     5341 gacctagcag accaactaat tcatctgcac tattttgatt gtttttcaga atctgctata
     5401 agaaatacca tattaggacg tatagttagt cctaggtgtg aatatcaagc aggacataac
     5461 aaggtaggat ctctacagta cttggcacta gcagcattaa taaaaccaaa acagataaag
     5521 ccacctttgc ctagtgttag gaaactgaca gaggacagat ggaacaagcc ccagaagacc
     5581 aagggccaca gagggagcca tacaatgaat ggacactaga gcttttagag gaacttaaga
     5641 gtgaagctgt tagacatttt cctaggatat ggctccataa cttaggacaa catatctatg
     5701 aaacttacgg ggatacttgg gcaggagtgg aagccataat aagaattctg caacaactgc
     5761 tgtttatcca tttcagaatt gggtgtcgac atagcagaat aggcgttact cgacagagga
     5821 gagcaagaaa tggagccagt agatcctaga ctagagccct ggaagcatcc aggaagtcag
     5881 cctaaaactg cttgtaccaa ttgctattgt aaaaagtgtt gctttcattg ccaagtttgt
     5941 ttcatgacaa aagccttagg catctcctat ggcaggaaga agcggagaca gcgacgaaga
     6001 gctcatcaga acagtcagac tcatcaagct tctctatcaa agcagtaagt agtacatgta
     6061 atgcaaccta taatagtagc aatagtagca ttagtagtag caataataat agcaatagtt
     6121 gtgtggtcca tagtaatcat agaatatagg aaaatattaa gacaaagaaa aatagacagg
     6181 ttaattgata gactaataga aagagcagaa gacagtggca atgagagtga aggagaagta
     6241 tcagcacttg tggagatggg ggtggaaatg gggcaccatg ctccttggga tattgatgat
     6301 ctgtagtgct acagaaaaat tgtgggtcac agtctattat ggggtacctg tgtggaagga
     6361 agcaaccacc actctatttt gtgcatcaga tgctaaagca tatgatacag aggtacataa
     6421 tgtttgggcc acacatgcct gtgtacccac agaccccaac ccacaagaag tagtattggt
     6481 aaatgtgaca gaaaatttta acatgtggaa aaatgacatg gtagaacaga tgcatgagga
     6541 tataatcagt ttatgggatc aaagcctaaa gccatgtgta aaattaaccc cactctgtgt
     6601 tagtttaaag tgcactgatt tgaagaatga tactaatacc aatagtagta gcgggagaat
     6661 gataatggag aaaggagaga taaaaaactg ctctttcaat atcagcacaa gcataagaga
     6721 taaggtgcag aaagaatatg cattctttta taaacttgat atagtaccaa tagataatac
     6781 cagctatagg ttgataagtt gtaacacctc agtcattaca caggcctgtc caaaggtatc
     6841 ctttgagcca attcccatac attattgtgc cccggctggt tttgcgattc taaaatgtaa
     6901 taataagacg ttcaatggaa caggaccatg tacaaatgtc agcacagtac aatgtacaca
     6961 tggaatcagg ccagtagtat caactcaact gctgttaaat ggcagtctag cagaagaaga
     7021 tgtagtaatt agatctgcca atttcacaga caatgctaaa accataatag tacagctgaa
     7081 cacatctgta gaaattaatt gtacaagacc caacaacaat acaagaaaaa gtatccgtat
     7141 ccagagggga ccagggagag catttgttac aataggaaaa ataggaaata tgagacaagc
     7201 acattgtaac attagtagag caaaatggaa tgccacttta aaacagatag ctagcaaatt
     7261 aagagaacaa tttggaaata ataaaacaat aatctttaag caatcctcag gaggggaccc
     7321 agaaattgta acgcacagtt ttaattgtgg aggggaattt ttctactgta attcaacaca
     7381 actgtttaat agtacttggt ttaatagtac ttggagtact gaagggtcaa ataacactga
     7441 aggaagtgac acaatcacac tcccatgcag aataaaacaa tttataaaca tgtggcagga
     7501 agtaggaaaa gcaatgtatg cccctcccat cagtggacaa attagatgtt catcaaatat
     7561 tactgggctg ctattaacaa gagatggtgg taataacaac aatgggtccg agatcttcag
     7621 acctggagga ggcgatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     7681 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     7741 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     7801 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctgatatagt
     7861 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     7921 agtctggggc atcaaacagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     7981 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     8041 ttggaatgct agttggagta ataaatctct ggaacagatt tggaataaca tgacctggat
     8101 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     8161 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     8221 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     8281 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     8341 gcagggatat tcaccattat cgtttcagac ccacctccca atcccgaggg gacccgacag
     8401 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     8461 gaacggatcc ttagcactta tctgggacga tctgcggagc ctgtgcctct tcagctacca
     8521 ccgcttgaga gacttactct tgattgtaac gaggattgtg gaacttctgg gacgcagggg
     8581 gtgggaagcc ctcaaatatt ggtggaatct cctacagtat tggagtcagg aactaaagaa
     8641 tagtgctgtt aacttgctca atgccacagc catagcagta gctgagggga cagatagggt
     8701 tatagaagta ttacaagcag cttatagagc tattcgccac atacctagaa gaataagaca
     8761 gggcttggaa aggattttgc tataagatgg gtggcaagtg gtcaaaaagt agtgtgattg
     8821 gatggcctgc tgtaagggaa agaatgagac gagctgagcc agcagcagat ggggtgggag
     8881 cagtatctcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctaaca
     8941 atgctgcttg tgcctggcta gaagcacaag aggaggaaga ggtgggtttt ccagtcacac
     9001 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     9061 aagaaaaggg gggactggaa gggctaattc actcccaaag aagacaagat atccttgatc
     9121 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     9181 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
     9241 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
     9301 gaatggatga ccctgagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
     9361 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgacat cgagcttgct
     9421 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
     9481 ggcgagccct cagatgctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
     9541 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     9601 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     9661 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcac ccaggaggta
     9721 gaggttgcag tgagccaaga tcgcgccact gcattccagc ctgggcaaga aaacaagact
     9781 gtctaaaata ataataataa gttaagggta ttaaatatat ttatacatgg aggtcataaa
     9841 aatatatata tttgggctgg gcgcagtggc tcacacctgc gcccggccct ttgggaggcc
     9901 gaggcaggtg gatcacctga gtttgggagt tccagaccag cctgaccaac atggagaaac
     9961 cccttctctg tgtattttta gtagatttta ttttatgtgt attttattca caggtatttc
    10021 tggaaaactg aaactgtttt tcctctactc tgataccaca agaatcatca gcacagagga
    10081 agacttctgt gatcaaatgt ggtgggagag ggaggttttc accagcacat gagcagtcag
    10141 ttctgccgca gactcggcgg gtgtccttcg gttcagttcc aacaccgcct gcctggagag
    10201 aggtcagacc acagggtgag ggctcagtcc ccaagacata aacacccaag acataaacac
    10261 ccaacaggtc caccccgcct gctgcccagg cagagccgat tcaccaagac gggaattagg
    10321 atagagaaag agtaagtcac acagagccgg ctgtgcggga gaacggagtt ctattatgac
    10381 tcaaatcagt ctccccaagc attcggggat cagagttttt aaggataact tagtgtgtag
    10441 ggggccagtg agttggagat gaaagcgtag ggagtcgaag gtgtcctttt gcgccgagtc
    10501 agttcctggg tgggggccac aagatcggat gagccagttt atcaatccgg gggtgccagc
    10561 tgatccatgg agtgcagggt ctgcaaaata tctcaagcac tgattgatct taggttttac
    10621 aatagtgatg ttaccccagg aacaatttgg ggaaggtcag aatcttgtag cctgtagctg
    10681 catgactcct aaaccataat ttcttttttg tttttttttt tttatttttg agacagggtc
    10741 tcactctgtc acctaggctg gagtgcagtg gtgcaatcac agctcactgc agcctcaacg
    10801 tcgtaagctc aagcgatcct cccacctcag cctgcctggt agctgagact acaagcgacg
    10861 ccccagttaa tttttgtatt tttggtagag gcagcgtttt gccgtgtggc cctggctggt
    10921 ctcgaactcc tgggctcaag tgatccagcc tcagcctccc aaagtgctgg gacaaccggg
    10981 gccagtcact gcacctggcc ctaaaccata atttctaatc ttttggctaa tttgttagtc
    11041 ctacaaaggc agtctagtcc ccaggcaaaa agggggtttg tttcgggaaa gggctgttac
    11101 tgtctttgtt tcaaactata aactaagttc ctcctaaact tagttcggcc tacacccagg
    11161 aatgaacaag gagagcttgg aggttagaag cacgatggaa ttggttaggt cagatctctt
    11221 tcactgtctg agttataatt ttgcaatggt ggttcaaaga ctgcccgctt ctgacaccag
    11281 tcgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
    11341 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
    11401 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
    11461 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    11521 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    11581 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    11641 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    11701 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    11761 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    11821 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    11881 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    11941 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
    12001 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    12061 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    12121 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    12181 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    12241 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    12301 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    12361 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    12421 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    12481 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    12541 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    12601 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    12661 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    12721 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    12781 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    12841 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    12901 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    12961 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    13021 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    13081 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    13141 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    13201 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    13261 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
    13321 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
    13381 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
    13441 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
    13501 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
    13561 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
    13621 gtgcgggcct cttcgctatt acgccagggg aggcagagat tgcagtaagc tgagatcgca
    13681 gcactgcact ccagcctggg cgacagagta agactctgtc tcaaaaataa aataaataaa
    13741 tcaatcagat attccaatct tttcctttat ttatttattt attttctatt ttggaaacac
    13801 agtccttcct tattccagaa ttacacatat attctatttt tctttatatg ctccagtttt
    13861 ttttagacct tcacctgaaa tgtgtgtata caaaatctag gccagtccag cagagcctaa
    13921 aggtaaaaaa taaaataata aaaaataaat aaaatctagc tcactccttc acatcaaaat
    13981 ggagatacag ctgttagcat taaataccaa ataacccatc ttgtcctcaa taattttaag
    14041 cgcctctctc caccacatct aactcctgtc aaaggcatgt gccccttccg ggcgctctgc
    14101 tgtgctgcca accaactggc atgtggactc tgcagggtcc ctaactgcca agccccacag
    14161 tgtgccctga ggctgcccct tccttctagc ggctgccccc actcggcttt gctttcccta
    14221 gtttcagtta cttgcgttca gccaaggtct gaaactaggt gcgcacagag cggtaagact
    14281 gcgagagaaa gagaccagct ttacaggggg tttatcacag tgcaccctga cagtcgtcag
    14341 cctcacaggg ggtttatcac attgcaccct gacagtcgtc agcctcacag ggggtttatc
    14401 acagtgcacc cttacaatca ttccatttga ttcacaattt ttttagtctc tactgtgcct
    14461 aacttgtaag ttaaatttga tcagaggtgt gttcccagag gggaaaacag tatatacagg
    14521 gttcagtact atcgcatttc aggcctccac ctgggtcttg gaatgtgtcc cccgaggggt
    14581 gatgactacc tcagttggat ctccacaggt cacagtgaca caagataacc aagacacctc
    14641 ccaaggctac cacaatgggc cgccctccac gtgcacatgg ccggaggaac tgccatgtcg
    14701 gaggtgcaag cacacctgcg catcagagtc cttggtgtgg agggagggac cagcgcagct
    14761 tccagccatc cacctgatga acagaaccta gggaaagccc cagttctact tacaccagga
    14821 aaggc
//

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