pNL4-3-Luc-R-E-

價格:12600元

聯系方式:I47-825O-882O

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pNL4.3-Luc HIV-1 NL4-3 ΔEnv Vpr Luciferase載體基本信息

出品公司: NIH
載體名稱: pNL4-3-Luc-R-E-、pNL4.3-Luc
pNL4-3-Luc、HIV-1 NL4-3 ΔEnv Vpr Luciferase Reporter Vector  
 
載體又名: HIV-1 NL4-3 ΔEnv Vpr Luciferase Reporter Vector 、 HIV-1 vector pNL4-3-Luc-R-E-、pNL4-3-Luc-R-E-載體、pNL4.3-Luc載體

HIV-1 NL4-3 ΔEnv Vpr Luciferase Reporter Vector (pNL4-3.Luc.R-E-)
質粒類型: 哺乳動物細胞載體;逆病毒包裝載體
高拷貝/低拷貝: 高拷貝
克隆方法: 限制性內切酶,多克隆位點
啟動子: CMV
載體大小: 16396 bp
5' 測序引物及序列: --
3' 測序引物及序列: --
存儲人: Dr. Nathaniel Landau, Aaron Diamond AIDS Research Center, The Rockefeller University.
載體抗性: 氨芐青霉素
篩選標記: 嘌呤霉素(Puromycin)
克隆菌株: DH5α 等
宿主細胞(系): 常規細胞系(293、CV-1、CHO等)
備注: pNL4-3-Luc-R-E-載體,又叫pNL4-3-Luc載體,是一個HIV-1 NL4-3熒光素酶報告質粒,包含有缺陷的Nef,  Env 和 Vpr.
這個質粒與含有env蛋白表達質粒共轉染哺乳細胞,能夠包裝產生病毒,但是該病毒不具有再次擴增能力,因子病毒只能由共轉染的質粒產生一輪病毒。
產品目錄號: 3418
穩定性: 穩表達
組成型/誘導型: 組成型
病毒/非病毒: 非病毒
生物危害性:
參考文獻:
He J, Choe S, Walker R, Di Marzio P, Morgan DO, Landau NR. Human immunodeficiency virus type 1 viral protein R (Vpr) arrests cells in the G2 phase of the cell cycle by inhibiting p34cdc2 activity. J Virol 69: 6705–6711, 1995.
 
Connor RI, Chen BK, Choe S, Landau NR. Vpr is required for efficient replication of human immunodeficiency virus type-1 in mononuclear phagocytes. Virology 206: 935–944, 1995.
 
文章引用格式: 文章引用部分可以這樣書寫:“The following reagent was obtained through the NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH: pNL4-3.Luc.R.E from Dr. Nathaniel Landau.” Also include the references cited above in any publications.
 
載體說明書:  pNL4-3-Luc-R-E-載體說明書
pNL4-3-Luc-R-E-載體使用方法
pNL4-3-Luc-R-E-載體圖譜和序列
 
相關載體: pNL4-3

pNL4.3-Luc HIV-1 NL4-3 ΔEnv Vpr Luciferase載體質粒圖譜和多克隆位點信息

pNL4-3-Luc-R-E載體圖譜

pNL4.3-Luc HIV-1 NL4-3 ΔEnv Vpr Luciferase載體簡介

pNL4.3-Luc HIV-1 NL4-3 ΔEnv Vpr Luciferase載體序列

LOCUS       pNL4-3-Luc-R-E-             16396 bp ds-DNA     circular SYN 26-MAR-2020
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, March 26, 2020 from SnapGene Viewer 3.2.1 to 
            Vector NTI(R) format
            http://www.vpiqnf.icu
COMMENT     VNTDATE|909100800|
COMMENT     VNTDBDATE|909100800|
COMMENT     VNTNAME|pNL4-3-luc|
FEATURES             Location/Qualifiers
     promoter        669..773
                     /gene="bla"
                     /label=AmpR promoter
     CDS             774..1634
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     rep_origin      1805..2393
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     LTR             4204..4837
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     CDS             complement(5027..6673)
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
     misc_feature    7486..7719
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             complement(10388..13399)
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
     misc_feature    10586..10703
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(13192..14694)
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
     LTR             14850..15107
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgctggactg gcctagattt tgtatacaca catttcaggt gaaggtctaa aaaaaactgg
       61 agcatataaa gaaaaataga atatatgtgt aattctggaa taaggaagga ctgtgtttcc
      121 aaaatagaaa ataaataaat aaataaagga aaagattgga atatctgatt gatttattta
      181 ttttattttt gagacagagt cttactctgt cgcccaggct ggagtgcagt gctgcgatct
      241 cagcttactg caatctctgc ctcccctggc gtaatagcga agaggcccgc accgatcgcc
      301 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
      361 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
      421 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
      481 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
      541 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
      601 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
      661 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      721 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
      781 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      841 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      901 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
      961 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     1021 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1081 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1141 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1201 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1261 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1321 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1381 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1441 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     1501 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1561 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1621 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1681 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1741 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1801 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1861 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1921 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     1981 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2041 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2101 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2161 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2221 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2281 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2341 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2401 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     2461 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2521 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2581 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc gactggtgtc
     2641 agaagcgggc agtctttgaa ccaccattgc aaaattataa ctcagacagt gaaagagatc
     2701 tgacctaacc aattccatcg tgcttctaac ctccaagctc tccttgttca ttcctgggtg
     2761 taggccgaac taagtttagg aggaacttag tttatagttt gaaacaaaga cagtaacagc
     2821 cctttcccga aacaaacccc ctttttgcct ggggactaga ctgcctttgt aggactaaca
     2881 aattagccaa aagattagaa attatggttt agggccaggt gcagtgactg gccccggttg
     2941 tcccagcact ttgggaggct gaggctggat cacttgagcc caggagttcg agaccagcca
     3001 gggccacacg gcaaaacgct gcctctacca aaaatacaaa aattaactgg ggcgtcgctt
     3061 gtagtctcag ctaccaggca ggctgaggtg ggaggatcgc ttgagcttac gacgttgagg
     3121 ctgcagtgag ctgtgattgc accactgcac tccagcctag gtgacagagt gagaccctgt
     3181 ctcaaaaata aaaaaaaaaa aacaaaaaag aaattatggt ttaggagtca tgcagctaca
     3241 ggctacaaga ttctgacctt ccccaaattg ttcctggggt aacatcacta ttgtaaaacc
     3301 taagatcaat cagtgcttga gatattttgc agaccctgca ctccatggat cagctggcac
     3361 ccccggattg ataaactggc tcatccgatc ttgtggcccc cacccaggaa ctgactcggc
     3421 gcaaaaggac accttcgact ccctacgctt tcatctccaa ctcactggcc ccctacacac
     3481 taagttatcc ttaaaaactc tgatccccga atgcttgggg agactgattt gagtcataat
     3541 agaactccgt tctcccgcac agccggctct gtgtgactta ctctttctct atcctaattc
     3601 ccgtcttggt gaatcggctc tgcctgggca gcaggcgggg tggacctgtt gggtgtttat
     3661 gtcttgggtg tttatgtctt ggggactgag ccctcaccct gtggtctgac ctctctccag
     3721 gcaggcggtg ttggaactga accgaaggac acccgccgag tctgcggcag aactgactgc
     3781 tcatgtgctg gtgaaaacct ccctctccca ccacatttga tcacagaagt cttcctctgt
     3841 gctgatgatt cttgtggtat cagagtagag gaaaaacagt ttcagttttc cagaaatacc
     3901 tgtgaataaa atacacataa aataaaatct actaaaaata cacagagaag gggtttctcc
     3961 atgttggtca ggctggtctg gaactcccaa actcaggtga tccacctgcc tcggcctccc
     4021 aaagggccgg gcgcaggtgt gagccactgc gcccagccca aatatatata tttttatgac
     4081 ctccatgtat aaatatattt aataccctta acttattatt attattttag acagtcttgt
     4141 tttcttgccc aggctggaat gcagtggcgc gatcttggct cactgcaacc tctacctcct
     4201 gggtgctaga gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt
     4261 cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa
     4321 gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag
     4381 acccagtaca ggcaaaaagc agctgcttat atgcagcatc tgagggctcg ccactcccca
     4441 gtcccgccca ggccacgcct ccctggaaag tccccagcgg aaagtccctt gtagcaagct
     4501 cgatgtcagc agttcttgaa gtactccgga tgcagctctc gggccacgtg atgaaatgct
     4561 aggcggctgt caaacctcca ctctaacact tctctctcag ggtcatccat tccatgcagg
     4621 ctcacagggt gtaacaagct ggtgttctct cctttattgg cctcttctac cttatctggc
     4681 tcaactggta ctagcttgta gcaccatcca aaggtcagtg gatatctgac ccctggccct
     4741 ggtgtgtagt tctgccaatc agggaagtag ccttgtgtgt ggtagatcca cagatcaagg
     4801 atatcttgtc ttctttggga gtgaattagc ccttccagtc cccccttttc ttttaaaaag
     4861 tggctaagat ctacagctgc cttgtaagtc attggtctta aaggtacctg aggtgtgact
     4921 ggaaaaccca cctcttcctc ctcttgtgct tctagccagg cacaagcagc attgttagct
     4981 gctgtattgc tacttgtgat tgctccatgt ttttctaggt ctcgagcaat ttggactttc
     5041 cgcccttctt ggcctttatg aggatctctc tgatttttct tgcgtcgagt tttccggtaa
     5101 gacctttcgg tacttcgtcc acaaacacaa ctcctccgcg caactttttc gcggttgtta
     5161 cttgactggc gacgtaatcc acgatctctt tttccgtcat cgtctttccg tgctccaaaa
     5221 caacaacggc ggcgggaagt tcaccggcgt catcgtcggg aagacctgcc acgcccgcgt
     5281 cgaagatgtt ggggtgttgt aacaatatcg attccaattc agcgggggcc acctgatatc
     5341 ctttgtattt aattaaagac ttcaagcggt caactatgaa gaagtgttcg tcttcgtccc
     5401 agtaagctat gtctccagaa tgtagccatc catccttgtc aatcaaggcg ttggtcgctt
     5461 ccggattgtt tacataaccg gacataatca taggtcctct gacacataat tcgcctctct
     5521 gattaacgcc cagcgttttc ccggtatcca gatccacaac cttcgcttca aaaaatggaa
     5581 caactttacc gaccgcgccc ggtttatcat ccccctcggg tgtaatcaga atagctgatg
     5641 tagtctcagt gagcccatat ccttgtcgta tccctggaag atggaagcgt tttgcaaccg
     5701 cttccccgac ttctttcgaa agaggtgcgc ccccagaagc aatttcgtgt aaattagata
     5761 aatcgtattt gtcaatcaga gtgcttttgg cgaagaatga aaatagggtt ggtactagca
     5821 acgcactttg aattttgtaa tcctgaaggg atcgtaaaaa cagctcttct tcaaatctat
     5881 acattaagac gactcgaaat ccacatatca aatatccgag tgtagtaaac attccaaaac
     5941 cgtgatggaa tggaacaaca cttaaaatcg cagtatccgg aatgatttga ttgccaaaaa
     6001 taggatctct ggcatgcgag aatctgacgc aggcagttct atgcggaagg gccacaccct
     6061 taggtaaccc agtagatcca gaggaattca ttatcagtgc aattgttttg tcacgatcaa
     6121 aggactctgg tacaaaatcg tattcattaa aaccgggagg tagatgagat gtgacgaacg
     6181 tgtacatcga ctgaaatccc tggtaatccg ttttagaatc catgataata attttctgga
     6241 ttattggtaa ttttttttgc acgttcaaaa ttttttgcaa cccctttttg gaaacaaaca
     6301 ctacggtagg ctgcgaaatg ttcatactgt tgagcaattc acgttcatta taaatgtcgt
     6361 tcgcgggcgc aactgcaact ccgataaata acgcgcccaa caccggcata aagaattgaa
     6421 gagagttttc actgcatacg acgattctgt gatttgtatt cagcccatat cgtttcatag
     6481 cttctgccaa ccgaacggac atttcgaagt attccgcgta cgtgatgttc acctcgatat
     6541 gtgcatctgt aaaagcaatt gttccaggaa ccagggcgta tctcttcata gccttatgca
     6601 gttgctctcc agcggttcca tcctctagag gatagaatgg cgccgggcct ttctttatgt
     6661 ttttggcgtc ttcagcggcc gcgccaccca tcttatagca aaatcctttc caagccctgt
     6721 cttattcttc taggtatgtg gcgaatagct ctacaagctc cttgtactac ttctataacc
     6781 ctatctgtcc cctcagctac tgctatggct gtggcattga gcaagctaac agcactattc
     6841 tttagctcct gactccaata ttgtaggaga ttccaccaat atttgagggc ttcccacccc
     6901 ctgcgtccca gaagttccac aatcctcgtt acaatcaaga gtaagtctct caagcggtgg
     6961 tagctgaaga ggcacaggct ccgcagatcg tcccagataa gtgctaagga tccgttcact
     7021 aatcgaatgg atctgtctct gtctctctct ccaccttctt cttctattcc ttcgggcctg
     7081 tcgggtcccc tcgggattgg gaggtgggtc tgaaacgata atggtgaata tccctgccta
     7141 actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact
     7201 atcattatga ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa
     7261 cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat
     7321 tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc
     7381 caggtcatgt tattccaaat ctgttccaga gatttattac tccaactagc attccaaggc
     7441 acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag ctgttgatcc
     7501 tttaggtatc tttccacagc caggattctt gcctggagct gtttgatgcc ccagactgtg
     7561 agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg ctgctgcact
     7621 atatcagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg
     7681 cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct
     7741 ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatttttact
     7801 actttatatt tatataattc acttctccaa ttgtccctca tatcgcctcc tccaggtctg
     7861 aagatctcgg acccattgtt gttattacca ccatctcttg ttaatagcag cccagtaata
     7921 tttgatgaac atctaatttg tccactgatg ggaggggcat acattgcttt tcctacttcc
     7981 tgccacatgt ttataaattg ttttattctg catgggagtg tgattgtgtc acttccttca
     8041 gtgttatttg acccttcagt actccaagta ctattaaacc aagtactatt aaacagttgt
     8101 gttgaattac agtagaaaaa ttcccctcca caattaaaac tgtgcgttac aatttctggg
     8161 tcccctcctg aggattgctt aaagattatt gttttattat ttccaaattg ttctcttaat
     8221 ttgctagcta tctgttttaa agtggcattc cattttgctc tactaatgtt acaatgtgct
     8281 tgtctcatat ttcctatttt tcctattgta acaaatgctc tccctggtcc cctctggata
     8341 cggatacttt ttcttgtatt gttgttgggt cttgtacaat taatttctac agatgtgttc
     8401 agctgtacta ttatggtttt agcattgtct gtgaaattgg cagatctaat tactacatct
     8461 tcttctgcta gactgccatt taacagcagt tgagttgata ctactggcct gattccatgt
     8521 gtacattgta ctgtgctgac atttgtacat ggtcctgttc cattgaacgt cttattatta
     8581 cattttagaa tcgcaaaacc agccggggca caataatgta tgggaattgg ctcaaaggat
     8641 acctttggac aggcctgtgt aatgactgag gtgttacaac ttatcaacct atagctggta
     8701 ttatctattg gtactatatc aagtttataa aagaatgcat attctttctg caccttatct
     8761 cttatgcttg tgctgatatt gaaagagcag ttttttatct ctcctttctc cattatcatt
     8821 ctcccgctac tactattggt attagtatca ttcttcaaat cagtgcactt taaactaaca
     8881 cagagtgggg ttaattttac acatggcttt aggctttgat cccataaact gattatatcc
     8941 tcatgcatct gttctaccat gtcatttttc cacatgttaa aattttctgt cacatttacc
     9001 aatactactt cttgtgggtt ggggtctgtg ggtacacagg catgtgtggc ccaaacatta
     9061 tgtacctctg tatcatatat gctttagcat ctgatgcaca aaatagagtg gtggttgctt
     9121 ccttccacac aggtacccca taatagactg tgacccacaa tttttctgta gcactacaga
     9181 tcatcaatat cccaaggagc atggtgcccc atttccaccc ccatctccac aagtgctgat
     9241 acttctcctt cactctcatt gccactgtct tctgctcttt ctattagtct atcaattaac
     9301 ctgtctattt ttctttgtct taatattttc ctatattcta tgattactat ggaccacaca
     9361 actattgcta ttattattgc tactactaat gctactattg ctactattat aggttgcatt
     9421 acatgtacta cttactgctt tgatagagaa gcttgatgag tctgactgtt ctgatgagct
     9481 cttcgtcgct gtctccgctt cttcctgcca taggagatgc ctaaggcttt tgtcatgaaa
     9541 caaacttggc aatgaaagca acacttttta caatagcaat tggtacaagc agttttaggc
     9601 tgacttcctg gatgcttcca gggctctagt ctaggatcta ctggctccat ttcttgctct
     9661 cctctgtcga gtaacgccta ttctgctatg tcgacaccca attctgaaat ggataaacag
     9721 cagttgttgc agaattctta ttatggcttc cactcctgcc caagtatccc cgtaagtttc
     9781 atagatatgt tgtcctaagt tatggagcca tatcctagga aaatgtctaa cagcttcact
     9841 cttaattaag ttcctctaaa agctctagtg tccattcatt gtatggctcc ctctgtggcc
     9901 cttggtcttc tggggcttgt tccatctgtc ctctgtcagt ttcctaacac taggcaaagg
     9961 tggctttatc tgttttggtt ttattaatgc tgctagtgcc aagtactgta gagatcctac
    10021 cttgttatgt cctgcttgat attcacacct aggactaact atacgtccta atatggtatt
    10081 tcttatagca gattctgaaa aacaatcaaa atagtgcaga tgaattagtt ggtctgctag
    10141 gtcagggtct acttgtgtgc tatatctctt tttcctccat tctatggaga ctccctgacc
    10201 caaatgccag tctctttctc ctgtatgcag accccaatat gttgttatta ctaatttagc
    10261 atcccctagt gggatgtgta cttctgaact tatttttgga ttagtacttt catagtgatg
    10321 tctataaaac cagtccttag ctttccttga aatatacata tggtgtttta ctaatctttt
    10381 ccatgtgtta atcctcatcc tgtctacttg ccacacaatc atcacctgcc atctgttttc
    10441 cataatccct gatgatcttt gcttttcttc ttggcactac ttttatgtca ctattatctt
    10501 gtattactac tgccccttca cctttccaga ggagctttgc tggtcctttc caaactggat
    10561 ctctgctgtc cctgtaataa acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt
    10621 ctttagtttg tatgtctgtt gctattatgt ctactattct ttcccctgca ctgtaccccc
    10681 caatcccccc ttttctttta aaattgtgga tgaatactgc catttgtact gctgtcttaa
    10741 gatgttcagc ctgatctctt acctgtccta taattttctt taattcttta ttcatagatt
    10801 ctattactcc ttgactttgg ggattgtagg gaatgccaaa ttcctgcttg atccccgccc
    10861 accaacaggc ggccttaact gtagtactgg tgaaattgct gccattgtct gtatgtactg
    10921 tttttactgg ccatcttcct gctaatttta agaggaagta tgctgtttct tgccctgtct
    10981 ctgctggaat tacttctgct tctatatatc cactggctac atgaactgct accaagataa
    11041 cttttccttc taaatgtgta caatctagct gccatattcc tgggctacag tctacttgtc
    11101 catgcatggc ttcccctttt agctgacatt tatcacagct ggctactatt tcttttgcta
    11161 ctacaggtgg taggttaaaa tcactagcca ttgctctcca attactgtga tatttctcat
    11221 gttcttcttg ggccttatct attccatcta aaaatagtac tttcctgatt ccagcactga
    11281 ccaatttatc tacttgttca tttcctccaa ttcctttgtg tgctggtacc catgccaggt
    11341 agactttttc cttttttatt aactgctcta ttatttgact gactaactct gattcactct
    11401 tatctggttg tgcttgaatg attcccaatg catattgtga gtctgtcact atgtttactt
    11461 ctaatcccga atcctgcaaa gctagatgaa ttgcttgtaa ctcagtcttc tgatttgttg
    11521 tgtccgttag ggggacaact ttttgtcttc ctctgtcagt tacatatcct gcttttccta
    11581 atttagtttc cctattggct gccccatcta catagaaagt ttctgctcct attatgggtt
    11641 ctttctctaa ctggtaccat aacttcacta agggaggggt attgacaaac tcccactcag
    11701 gaatccaggt ggcttgccaa tactctgtcc accatgcttc ccatgtttcc ttttgtatgg
    11761 gtaatttaaa tttaggagtc tttccccata ttactatgct ttctgtggct attttttgta
    11821 ctgcctctgt taattgtttc acatcattag tgtgggcacc cttcattctt gcatactttc
    11881 ctgttttcag atttttaaat ggctcttgat aaatttgata tgtccattgg ccttgcccct
    11941 gcttctgtat ttctgctatt aagtcttttg atgggtcata atacactcca tgtaccggtt
    12001 cttttagaat ctccctgttt tctgccagtt ctagctctgc ttcttctgtt agtggtacta
    12061 cttctgttag tgctttggtt cccctaagaa gtttacataa ttgccttact ttaatccctg
    12121 cataaatctg acttgcccaa ttcaattttc ccactaattt ctgtatgtca ttgacagtcc
    12181 agctgtcctt ttctggcagc actataggct gtactgtcca tttatcagga tggagttcat
    12241 aacccatcca aaggaatgga ggttctttct gatgtttttt gtctggtgtg gtaaatcccc
    12301 acctcaacag atgttgtctc agttcctcta tttttgttct atgctgccct atttctaagt
    12361 cagatcctac atacaaatca tccatgtatt gatagatgac tatgtctgga ttttgttttc
    12421 taaaaggctc taagattttt gtcatgctac actggaatat tgctggtgat cctttccatc
    12481 cctgtggaag cacattgtac tgatatctaa tccctggtgt ctcattgttt atactaggta
    12541 tggtaaatgc agtatacttc ctgaagtctt tatctaaggg aactgaaaaa tatgcatcgc
    12601 ccacatccag tactgttact gattttttct gttttaaccc tgcaggatgt ggtattccta
    12661 attgaacttc ccagaaatct tgagttctct tattaagttc tctgaaatct actaattttc
    12721 tccatttagt actgtctttt ttctttatgg caaatactgg agtattgtat ggattttcag
    12781 gcccaatttt tgaaattttt ccttcctttt ccatttctgt acaaatttct actaatgctt
    12841 ttattttttc ttctgtcaat ggccattgtt taacttttgg gccatccatt cctggcttta
    12901 attttactgg tacagtctca ataggactaa tgggaaaatt taaagtgcag ccaatctgag
    12961 tcaacagatt tcttccaatt atgttgacag gtgtaggtcc tactaatact gtacctatag
    13021 ctttatgtcc gcagatttct atgagtatct gatcatactg tcttactttg ataaaacctc
    13081 caattccccc tatcattttt ggtttccatc ttcctggcaa attcatttct tctaatactg
    13141 tatcatctgc tcctgtatct aatagagctt cctttaattg cccccctatc tttattgtga
    13201 cgaggggtcg ctgccaaaga gtgatctgag ggaagctaaa ggatacagtt ccttgtctat
    13261 cggctcctgc ttctgagagg gagttgttgt ctcttcccca aacctgaagc tctcttctgg
    13321 tggggctgtt ggctctggtc tgctctgaag aaaattccct ggccttccct tgtgggaagg
    13381 ccagatcttc cctaaaaaat tagcctgtct ctcagtacaa tctttcattt ggtgtccttc
    13441 ctttccacat ttccaacagc cctttttcct aggggccctg caatttttgg ctatgtgccc
    13501 ttctttgcca caattgaaac acttaacagt ctttctttgg ttcctaaaat tgcctttctg
    13561 tatcattatg gtagctggat ttgttacttg gctcattgct tcagccaaaa ctcttgcttt
    13621 atggccgggt ccccccactc cctgacatgc tgtcatcatt tcttctagtg tcgctcctgg
    13681 tcccaatgct tttaaaatag tcttacaatc tgggttcgca ttttggacca acaaggtttc
    13741 tgtcatccaa ttttttacct cttgtgaagc ttgctcggct cttagagttt tatagaatcg
    13801 gtctacatag tctctaaagg gttcctttgg tccttgtctt atgtccagaa tgctggtagg
    13861 gctatacatt cttactattt tatttaatcc caggattatc catcttttat agatttctcc
    13921 tactgggata ggtggattat gtgtcatcca tcctatttgt tcctgaaggg tactagtagt
    13981 tcctgctatg tcacttcccc ttggttctct catctggcct ggtgcaatag gccctgcatg
    14041 cactggatgc aatctatccc attctgcagc ttcctcattg atggtctctt ttaacatttg
    14101 catggctgct tgatgtcccc ccactgtgtt tagcatggta tttaaatctt gtggggtggc
    14161 tccttctgat aatgctgaaa acatgggtat tacttctggg ctgaaagcct tctcttctac
    14221 tacttttacc catgcattta aagttctagg tgatatggcc tgatgtacca tttgcccctg
    14281 gaggttctgc actatagggt aattttggct gacctggctg ttgtttcctg tgtcagctgc
    14341 tgcttgctgt gcctttttct tacttttgtt ttgctcttcc tctatcttat ctaaggcttc
    14401 cttggtgtct tttacatcta tcctttgatg cacacaatag aggactgcta ttgtattata
    14461 taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc ccagtatttg
    14521 tctacagcct tctgatgtct ctaaaaggcc aggattaact gcgaatcgtt ctagctccct
    14581 gcttgcccat actatatgtt ttagtttata ttgtttcttt ccccctggcc ttaaccgaat
    14641 tttttcccat ttatctaatt ctcccccgct taataccgac gctctcgcac ccatctctct
    14701 ccttctagcc tccgctagtc aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc
    14761 ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga gatctcctct ggctttactt
    14821 tcgctttcaa gtccctgttc gggcgccact gctagagatt ttccacactg actaaaaggg
    14881 tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta ctttgagcac
    14941 tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
    15001 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagct gcttatatgt
    15061 agcatctgag ggctcgccac tccccagtcc cgcccaggcc acacctccct ggaaagtccc
    15121 cagcggaaag tcccttgtag aaagctcgat gtcagcagtc tttgtagtac tccggatgca
    15181 gctctcgggc catgtgacga aatgctagga ggctgtcaaa cttccacact aatacttctc
    15241 cctccgggtc ctccatccca tgctggctca tagggtgtaa caagctgttg ttctctcctt
    15301 cattggcctc ttctacttgc tctggttcaa ctggtactaa cttgaagcac catccaaagg
    15361 tcagtggata tctgatccct ggccctggtg tgtagttctg ccaatcaggg aagtagcctt
    15421 gtgtgtggta gatccacaga tcaaggatct cttgtctttt ttgggaccaa attagccctt
    15481 ccagcctttc ctggtgtaag tagaactggg gctttcccta ggttctgttc atcaggtgga
    15541 tggctggaag ctgcgctggt ccctccctcc acaccaagga ctctgatgcg caggtgtgct
    15601 tgcacctccg acatggcagt tcctccggcc atgtgcacgt ggagggcggc ccattgtggt
    15661 agccttggga ggtgtcttgg ttatcttgtg tcactgtgac ctgtggagat ccaactgagg
    15721 tagtcatcac ccctcggggg acacattcca agacccaggt ggaggcctga aatgcgatag
    15781 tactgaaccc tgtatatact gttttcccct ctgggaacac acctctgatc aaatttaact
    15841 tacaagttag gcacagtaga gactaaaaaa attgtgaatc aaatggaatg attgtaaggg
    15901 tgcactgtga taaaccccct gtgaggctga cgactgtcag ggtgcaatgt gataaacccc
    15961 ctgtgaggct gacgactgtc agggtgcact gtgataaacc ccctgtaaag ctggtctctt
    16021 tctctcgcag tcttaccgct ctgtgcgcac ctagtttcag accttggctg aacgcaagta
    16081 actgaaacta gggaaagcaa agccgagtgg gggcagccgc tagaaggaag gggcagcctc
    16141 agggcacact gtggggcttg gcagttaggg accctgcaga gtccacatgc cagttggttg
    16201 gcagcacagc agagcgcccg gaaggggcac atgcctttga caggagttag atgtggtgga
    16261 gagaggcgct taaaattatt gaggacaaga tgggttattt ggtatttaat gctaacagct
    16321 gtatctccat tttgatgtga aggagtgagc tagattttat ttatttttta ttattttatt
    16381 ttttaccttt aggctc
//

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