PX391

價格:1000元

聯系方式:I47-825O-882O

買家導航

載體基本信息

出品公司: Addgene
載體名稱: PX391 或 PX391 Sphaerochaeta globus str. Buddy Cas9
質粒類型: CRISPR-Cas9載體;基因敲除載體;昆蟲細胞載體
高拷貝/低拷貝: 高拷貝
插入位點: 限制性內切酶,多克隆位點
啟動子: CMV
載體大小: 10733 bp
5' 測序引物及序列: CMV Forward:CGCAAATGGGCGGTAGGCGTG
3' 測序引物及序列: C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3')
載體標簽: NLS (C ter);HA、NLS (N ter)
載體抗性: 氨芐青霉素
篩選標記: Neomycin
克隆菌株: Stbl3
宿主細胞(系): 昆蟲細胞
備注: --
產品目錄號: #68328
穩定性: 穩表達
組成型/誘導型: 組成型
病毒/非病毒: 桿狀病毒

載體質粒圖譜和多克隆位點信息

PX391載體圖譜



載體簡介

載體序列

LOCUS       PX391 Sphaerochaeta globus str. Buddy Cas9	10733 bp 	DNA	SYN
DEFINITION  PX391 Sphaerochaeta globus str. Buddy Cas9
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..10733
                     /organism="PX391 Sphaerochaeta globus str. Buddy Cas9"
                     /mol_type="other DNA"
     misc_feature    47..1001
                     /label="Ac_5_flank"
     CDS             complement(343..948)
                     /label="ORF frame 3"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1023..1587
                     /label="CMV_immearly_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1090..1377
                     /label="CAG_enhancer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1544..1564
                     /label="CMV_fwd_primer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1615..1633
                     /label="T7_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1633..1656
                     /label="lacO"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1772..1796
                     /label="attB1"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     CDS             1822..5424
                     /label="ORF frame 1"
                     /translation="MYPYDVPDYASPKKKRKVEASMSKKVSRRYEEQAQEICQRLGSR
                     PYSIGLDLGVGSIGVAVAAYDPIKKQPSDLVFVSSRIFIPSTGAAERRQKRGQRNSLR
                     HRANRLKFLWKLLAERNLMLSYSEQDVPDPARLRFEDAVVRANPYELRLKGLNEQLTL
                     SELGYALYHIANHRGSSSVRTFLDEEKSSDDKKLEEQQAMTEQLAKEKGISTFIEVLT
                     AFNTNGLIGYRNSESVKSKGVPVPTRDIISNEIDVLLQTQKQFYQEILSDEYCDRIVS
                     AILFENEKIVPEAGCCPYFPDEKKLPRCHFLNEERRLWEAINNARIKMPMQEGAAKRY
                     QSASFSDEQRHILFHIARSGTDITPKLVQKEFPALKTSIIVLQGKEKAIQKIAGFRFR
                     RLEEKSFWKRLSEEQKDDFFSAWTNTPDDKRLSKYLMKHLLLTENEVVDALKTVSLIG
                     DYGPIGKTATQLLMKHLEDGLTYTEALERGMETGEFQELSVWEQQSLLPYYGQILTGS
                     TQALMGKYWHSAFKEKRDSEGFFKPNTNSDEEKYGRIANPVVHQTLNELRKLMNELIT
                     ILGAKPQEITVELARELKVGAEKREDIIKQQTKQEKEAVLAYSKYCEPNNLDKRYIER
                     FRLLEDQAFVCPYCLEHISVADIAAGRADVDHIFPRDDTADNSYGNKVVAHRQCNDIK
                     GKRTPYAAFSNTSAWGPIMHYLDETPGMWRKRRKFETNEEEYAKYLQSKGFVSRFESD
                     NSYIAKAAKEYLRCLFNPNNVTAVGSLKGMETSILRKAWNLQGIDDLLGSRHWSKDAD
                     TSPTMRKNRDDNRHHGLDAIVALYCSRSLVQMINTMSEQGKRAVEIEAMIPIPGYASE
                     PNLSFEAQRELFRKKILEFMDLHAFVSMKTDNDANGALLKDTVYSILGADTQGEDLVF
                     VVKKKIKDIGVKIGDYEEVASAIRGRITDKQPKWYPMEMKDKIEQLQSKNEAALQKYK
                     ESLVQAAAVLEESNRKLIESGKKPIQLSEKTISKKALELVGGYYYLISNNKRTKTFVV
                     KEPSNEVKGFAFDTGSNLCLDFYHDAQGKLCGEIIRKIQAMNPSYKPAYMKQGYSLYV
                     RLYQGDVCELRASDLTEAESNLAKTTHVRLPNAKPGRTFVIIITFTEMGSGYQIYFSN
                     LAKSKKGQDTSFTLTTIKNYDVRKVQLSSAGLVRYVSPLLVDKIEKDEVALCGE*"
     misc_feature    1825..1851
                     /label="HA"
                     /translation="MYPYDVPDYASPKKKRKVEASMSKKVSRRYEEQAQEICQRLGSR
                     PYSIGLDLGVGSIGVAVAAYDPIKKQPSDLVFVSSRIFIPSTGAAERRQKRGQRNSLR
                     HRANRLKFLWKLLAERNLMLSYSEQDVPDPARLRFEDAVVRANPYELRLKGLNEQLTL
                     SELGYALYHIANHRGSSSVRTFLDEEKSSDDKKLEEQQAMTEQLAKEKGISTFIEVLT
                     AFNTNGLIGYRNSESVKSKGVPVPTRDIISNEIDVLLQTQKQFYQEILSDEYCDRIVS
                     AILFENEKIVPEAGCCPYFPDEKKLPRCHFLNEERRLWEAINNARIKMPMQEGAAKRY
                     QSASFSDEQRHILFHIARSGTDITPKLVQKEFPALKTSIIVLQGKEKAIQKIAGFRFR
                     RLEEKSFWKRLSEEQKDDFFSAWTNTPDDKRLSKYLMKHLLLTENEVVDALKTVSLIG
                     DYGPIGKTATQLLMKHLEDGLTYTEALERGMETGEFQELSVWEQQSLLPYYGQILTGS
                     TQALMGKYWHSAFKEKRDSEGFFKPNTNSDEEKYGRIANPVVHQTLNELRKLMNELIT
                     ILGAKPQEITVELARELKVGAEKREDIIKQQTKQEKEAVLAYSKYCEPNNLDKRYIER
                     FRLLEDQAFVCPYCLEHISVADIAAGRADVDHIFPRDDTADNSYGNKVVAHRQCNDIK
                     GKRTPYAAFSNTSAWGPIMHYLDETPGMWRKRRKFETNEEEYAKYLQSKGFVSRFESD
                     NSYIAKAAKEYLRCLFNPNNVTAVGSLKGMETSILRKAWNLQGIDDLLGSRHWSKDAD
                     TSPTMRKNRDDNRHHGLDAIVALYCSRSLVQMINTMSEQGKRAVEIEAMIPIPGYASE
                     PNLSFEAQRELFRKKILEFMDLHAFVSMKTDNDANGALLKDTVYSILGADTQGEDLVF
                     VVKKKIKDIGVKIGDYEEVASAIRGRITDKQPKWYPMEMKDKIEQLQSKNEAALQKYK
                     ESLVQAAAVLEESNRKLIESGKKPIQLSEKTISKKALELVGGYYYLISNNKRTKTFVV
                     KEPSNEVKGFAFDTGSNLCLDFYHDAQGKLCGEIIRKIQAMNPSYKPAYMKQGYSLYV
                     RLYQGDVCELRASDLTEAESNLAKTTHVRLPNAKPGRTFVIIITFTEMGSGYQIYFSN
                     LAKSKKGQDTSFTLTTIKNYDVRKVQLSSAGLVRYVSPLLVDKIEKDEVALCGE*"
     misc_feature    1855..1875
                     /label="SV40 NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMSKKVSRRYEEQAQEICQRLGSR
                     PYSIGLDLGVGSIGVAVAAYDPIKKQPSDLVFVSSRIFIPSTGAAERRQKRGQRNSLR
                     HRANRLKFLWKLLAERNLMLSYSEQDVPDPARLRFEDAVVRANPYELRLKGLNEQLTL
                     SELGYALYHIANHRGSSSVRTFLDEEKSSDDKKLEEQQAMTEQLAKEKGISTFIEVLT
                     AFNTNGLIGYRNSESVKSKGVPVPTRDIISNEIDVLLQTQKQFYQEILSDEYCDRIVS
                     AILFENEKIVPEAGCCPYFPDEKKLPRCHFLNEERRLWEAINNARIKMPMQEGAAKRY
                     QSASFSDEQRHILFHIARSGTDITPKLVQKEFPALKTSIIVLQGKEKAIQKIAGFRFR
                     RLEEKSFWKRLSEEQKDDFFSAWTNTPDDKRLSKYLMKHLLLTENEVVDALKTVSLIG
                     DYGPIGKTATQLLMKHLEDGLTYTEALERGMETGEFQELSVWEQQSLLPYYGQILTGS
                     TQALMGKYWHSAFKEKRDSEGFFKPNTNSDEEKYGRIANPVVHQTLNELRKLMNELIT
                     ILGAKPQEITVELARELKVGAEKREDIIKQQTKQEKEAVLAYSKYCEPNNLDKRYIER
                     FRLLEDQAFVCPYCLEHISVADIAAGRADVDHIFPRDDTADNSYGNKVVAHRQCNDIK
                     GKRTPYAAFSNTSAWGPIMHYLDETPGMWRKRRKFETNEEEYAKYLQSKGFVSRFESD
                     NSYIAKAAKEYLRCLFNPNNVTAVGSLKGMETSILRKAWNLQGIDDLLGSRHWSKDAD
                     TSPTMRKNRDDNRHHGLDAIVALYCSRSLVQMINTMSEQGKRAVEIEAMIPIPGYASE
                     PNLSFEAQRELFRKKILEFMDLHAFVSMKTDNDANGALLKDTVYSILGADTQGEDLVF
                     VVKKKIKDIGVKIGDYEEVASAIRGRITDKQPKWYPMEMKDKIEQLQSKNEAALQKYK
                     ESLVQAAAVLEESNRKLIESGKKPIQLSEKTISKKALELVGGYYYLISNNKRTKTFVV
                     KEPSNEVKGFAFDTGSNLCLDFYHDAQGKLCGEIIRKIQAMNPSYKPAYMKQGYSLYV
                     RLYQGDVCELRASDLTEAESNLAKTTHVRLPNAKPGRTFVIIITFTEMGSGYQIYFSN
                     LAKSKKGQDTSFTLTTIKNYDVRKVQLSSAGLVRYVSPLLVDKIEKDEVALCGE*"
     misc_feature    1861..1875
                     /label="NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMSKKVSRRYEEQAQEICQRLGSR
                     PYSIGLDLGVGSIGVAVAAYDPIKKQPSDLVFVSSRIFIPSTGAAERRQKRGQRNSLR
                     HRANRLKFLWKLLAERNLMLSYSEQDVPDPARLRFEDAVVRANPYELRLKGLNEQLTL
                     SELGYALYHIANHRGSSSVRTFLDEEKSSDDKKLEEQQAMTEQLAKEKGISTFIEVLT
                     AFNTNGLIGYRNSESVKSKGVPVPTRDIISNEIDVLLQTQKQFYQEILSDEYCDRIVS
                     AILFENEKIVPEAGCCPYFPDEKKLPRCHFLNEERRLWEAINNARIKMPMQEGAAKRY
                     QSASFSDEQRHILFHIARSGTDITPKLVQKEFPALKTSIIVLQGKEKAIQKIAGFRFR
                     RLEEKSFWKRLSEEQKDDFFSAWTNTPDDKRLSKYLMKHLLLTENEVVDALKTVSLIG
                     DYGPIGKTATQLLMKHLEDGLTYTEALERGMETGEFQELSVWEQQSLLPYYGQILTGS
                     TQALMGKYWHSAFKEKRDSEGFFKPNTNSDEEKYGRIANPVVHQTLNELRKLMNELIT
                     ILGAKPQEITVELARELKVGAEKREDIIKQQTKQEKEAVLAYSKYCEPNNLDKRYIER
                     FRLLEDQAFVCPYCLEHISVADIAAGRADVDHIFPRDDTADNSYGNKVVAHRQCNDIK
                     GKRTPYAAFSNTSAWGPIMHYLDETPGMWRKRRKFETNEEEYAKYLQSKGFVSRFESD
                     NSYIAKAAKEYLRCLFNPNNVTAVGSLKGMETSILRKAWNLQGIDDLLGSRHWSKDAD
                     TSPTMRKNRDDNRHHGLDAIVALYCSRSLVQMINTMSEQGKRAVEIEAMIPIPGYASE
                     PNLSFEAQRELFRKKILEFMDLHAFVSMKTDNDANGALLKDTVYSILGADTQGEDLVF
                     VVKKKIKDIGVKIGDYEEVASAIRGRITDKQPKWYPMEMKDKIEQLQSKNEAALQKYK
                     ESLVQAAAVLEESNRKLIESGKKPIQLSEKTISKKALELVGGYYYLISNNKRTKTFVV
                     KEPSNEVKGFAFDTGSNLCLDFYHDAQGKLCGEIIRKIQAMNPSYKPAYMKQGYSLYV
                     RLYQGDVCELRASDLTEAESNLAKTTHVRLPNAKPGRTFVIIITFTEMGSGYQIYFSN
                     LAKSKKGQDTSFTLTTIKNYDVRKVQLSSAGLVRYVSPLLVDKIEKDEVALCGE*"
     CDS             complement(3289..3789)
                     /label="ORF frame 3"
                     /translation="MIHVCPSSCDICDANVLQTVRAHKGLILQQAEPFNIPFVQIVGL
                     AVLAICQHSLLFLFGLLLNDVLSFLSSDLQLSCQFHCDLLRFSSQDGNQLIHQLAQFV
                     QSLMDHWVGNPAVFFLIAICVRLKESLTVSLFFKSRVPILPHQGLSRSCQDLPIVGQQ
                     ALLLPH*"
     CDS             complement(4047..4589)
                     /label="ORF frame 1"
                     /translation="MSLIFFLTTNTRSSPWVSAPRIEYTVSFSRAPFASLSVFILTKA
                     CRSMNSRIFFLNSSRWASKDRFGSDAYPGIGIIASISTARLPCSDMVLIIWTRDLEQY
                     RATMASRPWCRLSSRFLRIVGLVSASLLQCRLPSRSSIPCRFQAFRRIDVSIPFRDPT
                     AVTLFGLNRQRRYSFAAFAI*"
     misc_feature    complement(5457..5474)
                     /label="Sp6_primer"
                     /translation="MSLIFFLTTNTRSSPWVSAPRIEYTVSFSRAPFASLSVFILTKA
                     CRSMNSRIFFLNSSRWASKDRFGSDAYPGIGIIASISTARLPCSDMVLIIWTRDLEQY
                     RATMASRPWCRLSSRFLRIVGLVSASLLQCRLPSRSSIPCRFQAFRRIDVSIPFRDPT
                     AVTLFGLNRQRRYSFAAFAI*"
     misc_feature    5479..5503
                     /label="attB2"
                     /translation="MSLIFFLTTNTRSSPWVSAPRIEYTVSFSRAPFASLSVFILTKA
                     CRSMNSRIFFLNSSRWASKDRFGSDAYPGIGIIASISTARLPCSDMVLIIWTRDLEQY
                     RATMASRPWCRLSSRFLRIVGLVSASLLQCRLPSRSSIPCRFQAFRRIDVSIPFRDPT
                     AVTLFGLNRQRRYSFAAFAI*"
     terminator      5515..5981
                     /label="hGH_PA_terminator"
                     /translation="MSLIFFLTTNTRSSPWVSAPRIEYTVSFSRAPFASLSVFILTKA
                     CRSMNSRIFFLNSSRWASKDRFGSDAYPGIGIIASISTARLPCSDMVLIIWTRDLEQY
                     RATMASRPWCRLSSRFLRIVGLVSASLLQCRLPSRSSIPCRFQAFRRIDVSIPFRDPT
                     AVTLFGLNRQRRYSFAAFAI*"
     terminator      5987..6022
                     /label="T7_terminator"
                     /translation="MSLIFFLTTNTRSSPWVSAPRIEYTVSFSRAPFASLSVFILTKA
                     CRSMNSRIFFLNSSRWASKDRFGSDAYPGIGIIASISTARLPCSDMVLIIWTRDLEQY
                     RATMASRPWCRLSSRFLRIVGLVSASLLQCRLPSRSSIPCRFQAFRRIDVSIPFRDPT
                     AVTLFGLNRQRRYSFAAFAI*"
     rep_origin      6139..6445
                     /label="f1_origin"
                     /translation="MSLIFFLTTNTRSSPWVSAPRIEYTVSFSRAPFASLSVFILTKA
                     CRSMNSRIFFLNSSRWASKDRFGSDAYPGIGIIASISTARLPCSDMVLIIWTRDLEQY
                     RATMASRPWCRLSSRFLRIVGLVSASLLQCRLPSRSSIPCRFQAFRRIDVSIPFRDPT
                     AVTLFGLNRQRRYSFAAFAI*"
     misc_feature    complement(6559..6579)
                     /label="pBABE_3_primer"
                     /translation="MSLIFFLTTNTRSSPWVSAPRIEYTVSFSRAPFASLSVFILTKA
                     CRSMNSRIFFLNSSRWASKDRFGSDAYPGIGIIASISTARLPCSDMVLIIWTRDLEQY
                     RATMASRPWCRLSSRFLRIVGLVSASLLQCRLPSRSSIPCRFQAFRRIDVSIPFRDPT
                     AVTLFGLNRQRRYSFAAFAI*"
     misc_feature    complement(6565..6780)
                     /label="SV40_enhancer"
                     /translation="MSLIFFLTTNTRSSPWVSAPRIEYTVSFSRAPFASLSVFILTKA
                     CRSMNSRIFFLNSSRWASKDRFGSDAYPGIGIIASISTARLPCSDMVLIIWTRDLEQY
                     RATMASRPWCRLSSRFLRIVGLVSASLLQCRLPSRSSIPCRFQAFRRIDVSIPFRDPT
                     AVTLFGLNRQRRYSFAAFAI*"
     promoter        6577..6845
                     /label="SV40_promoter"
                     /translation="MSLIFFLTTNTRSSPWVSAPRIEYTVSFSRAPFASLSVFILTKA
                     CRSMNSRIFFLNSSRWASKDRFGSDAYPGIGIIASISTARLPCSDMVLIIWTRDLEQY
                     RATMASRPWCRLSSRFLRIVGLVSASLLQCRLPSRSSIPCRFQAFRRIDVSIPFRDPT
                     AVTLFGLNRQRRYSFAAFAI*"
     rep_origin      6744..6821
                     /label="SV40_origin"
                     /translation="MSLIFFLTTNTRSSPWVSAPRIEYTVSFSRAPFASLSVFILTKA
                     CRSMNSRIFFLNSSRWASKDRFGSDAYPGIGIIASISTARLPCSDMVLIIWTRDLEQY
                     RATMASRPWCRLSSRFLRIVGLVSASLLQCRLPSRSSIPCRFQAFRRIDVSIPFRDPT
                     AVTLFGLNRQRRYSFAAFAI*"
     misc_feature    6806..6825
                     /label="SV40pro_F_primer"
                     /translation="MSLIFFLTTNTRSSPWVSAPRIEYTVSFSRAPFASLSVFILTKA
                     CRSMNSRIFFLNSSRWASKDRFGSDAYPGIGIIASISTARLPCSDMVLIIWTRDLEQY
                     RATMASRPWCRLSSRFLRIVGLVSASLLQCRLPSRSSIPCRFQAFRRIDVSIPFRDPT
                     AVTLFGLNRQRRYSFAAFAI*"
     CDS             6960..7754
                     /label="ORF frame 3"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            6963..7751
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(7269..7805)
                     /label="ORF frame 1"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      7931..8050
                     /label="SV40_PA_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    8019..8038
                     /label="EBV_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      8241..8276
                     /label="T7_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    complement(8299..8316)
                     /label="Baculovirs_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    8306..8383
                     /label="3_AcPH"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     CDS             complement(8413..9030)
                     /label="ORF frame 3"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     misc_feature    9010..9043
                     /label="loxP"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     gene            complement(9811..10671)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     CDS             complement(9811..10671)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
   61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
  121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
  181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
  241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
  301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
  361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
  421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
  481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
  541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
  601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
  661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
  721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
  781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
  841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
  901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
  961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
 1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
 1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
 1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
 1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
 1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
 1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
 1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
 1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
 1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
 1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
 1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
 1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
 1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
 1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
 1861 aaaaagcgca aggtcgaagc gtccatgagc aagaaagtca gtagacgcta tgaagaacag
 1921 gcacaggaga tttgtcagag gctggggagt agaccttatt ccatcgggct ggacctggga
 1981 gtgggatcta tcggagtggc agtcgccgct tacgacccaa ttaagaaaca gccctccgat
 2041 ctggtgttcg tcagctccag gatttttatc ccttctaccg gcgcagccga gcggagacag
 2101 aagagaggac agaggaacag cctgcgccac cgagcaaatc gcctgaaatt cctgtggaag
 2161 ctgctggctg aacgaaacct gatgctgtca tatagcgagc aggacgtgcc agatcctgca
 2221 cggctgagat ttgaggacgc tgtggtccga gcaaacccat acgagctgcg gctgaagggc
 2281 ctgaatgaac agctgaccct gagcgagctg gggtatgcac tgtaccacat cgccaatcat
 2341 aggggatcta gttcagtgcg cacattcctg gatgaggaaa agagctccga cgataagaaa
 2401 ctggaggaac agcaggctat gacagaacag ctggcaaaag agaagggaat ttccactttc
 2461 atcgaagtgc tgacagcctt taacactaat ggcctgatcg ggtacaggaa ctccgagtct
 2521 gtgaagagta agggcgtgcc agtccccact cgcgacatca tttcaaatga gattgatgtg
 2581 ctgctgcaga cccagaagca gttctatcag gaaatcctgt cagacgagta ctgcgatcgg
 2641 attgtcagcg caatcctgtt tgaaaacgag aagatcgtgc cagaagccgg ctgctgtccc
 2701 tatttccctg acgagaagaa actgcccaga tgtcactttc tgaatgagga aaggcgcctg
 2761 tgggaagcca ttaacaatgc taggatcaag atgcccatgc aggagggcgc tgcaaaacgc
 2821 taccagagtg cttcattcag cgacgagcag agacacattc tgtttcatat cgcaaggagc
 2881 gggactgata tcacccctaa actggtgcag aaggagttcc cagccctgaa aacctccatc
 2941 attgtgctgc agggaaaaga gaaggctatt cagaagatcg caggcttccg atttcgacgg
 3001 ctggaggaaa aatctttttg gaagagactg agtgaggaac agaaggacga tttctttagc
 3061 gcctggacaa acactcctga cgataaaaga ctgtccaagt acctgatgaa acacctgctg
 3121 ctgacagaaa atgaggtggt cgacgccctg aaaaccgtga gcctgattgg agattatggc
 3181 ccaatcggca agaccgcaac acagctgctg atgaaacatc tggaggatgg cctgacttac
 3241 accgaagccc tggagcgggg aatggaaacc ggcgagttcc aggaactgtc agtgtgggag
 3301 cagcagagcc tgctgcccta ctatgggcag atcctgacag gatctactca ggccctgatg
 3361 gggaagtatt ggcactctgc ttttaaagaa aagagagaca gtgagggatt ctttaagcct
 3421 aacacaaata gcgatgagga aaaatacggc aggattgcca acccagtggt ccatcagact
 3481 ctgaacgaac tgcgcaagct gatgaatgag ctgattacca tcctgggagc taaacctcag
 3541 gagatcacag tggaactggc acgagagctg aaggtcggag ctgagaaaag agaggacatc
 3601 attaagcagc agaccaaaca ggaaaaggag gctgtgctgg catatagcaa gtactgcgag
 3661 cccaacaatc tggacaaaag gtatattgaa aggttccgcc tgctggagga tcaggccttt
 3721 gtgtgccctt actgtctgga gcacattagc gtcgcagata tcgcagctgg aagggcagac
 3781 gtggatcata tcttcccacg cgacgataca gctgacaact cctacgggaa taaggtggtc
 3841 gcacaccgac agtgtaacga tatcaaggga aagcggaccc cctatgcagc cttcagtaat
 3901 acatcagcct ggggccccat catgcattac ctggacgaaa cccctgggat gtggcgcaaa
 3961 agaaggaagt ttgagacaaa cgaggaagag tatgctaagt acctgcagtc aaaaggcttc
 4021 gtgagcaggt ttgaaagcga taactcctat atcgcaaaag ctgcaaagga gtacctgcgc
 4081 tgcctgttca atccaaacaa tgtgactgcc gtcgggtccc tgaagggaat ggagacatct
 4141 atcctgcgga aggcctggaa tctgcaggga attgacgatc tgctgggcag ccggcactgg
 4201 agtaaggacg ccgataccag ccccacaatg cgcaaaaacc gggacgacaa tcggcaccat
 4261 ggcctggacg ccatcgtggc tctgtattgt tccagatctc tggtccagat gattaacacc
 4321 atgtccgagc agggcaagcg agcagtggaa atcgaggcta tgattcctat cccagggtac
 4381 gcatccgaac caaatctgtc tttcgaagcc cagcgggagc tgtttagaaa gaaaatcctg
 4441 gagttcatgg acctgcacgc ctttgtgagt atgaaaaccg acaacgatgc aaatggcgcc
 4501 ctgctgaaag atactgtgta ttcaattctg ggagcagaca cccagggaga ggatctggtg
 4561 ttcgtggtca agaaaaagat taaggacatc ggcgtgaaaa tcggggatta tgaagaggtc
 4621 gcatctgcca ttcgaggccg gatcaccgac aaacagccaa agtggtatcc catggaaatg
 4681 aaagataaga tcgagcagct gcagtctaag aacgaagccg ctctgcagaa atacaaggag
 4741 agtctggtgc aggcagccgc tgtcctggaa gagagtaata ggaagctgat tgagtcaggc
 4801 aaaaagccca tccagctgag tgaaaaaaca atttcaaaaa aggccctgga gctggtgggc
 4861 gggtactatt acctgattag caacaacaag cgcacaaaga ctttcgtggt caaggaacct
 4921 tcaaacgagg tgaaagggtt cgcatttgac actggaagca atctgtgcct ggacttttat
 4981 cacgatgccc agggaaagct gtgtggcgag atcattagaa aaatccaggc tatgaaccct
 5041 tcctataagc cagcatacat gaaacagggg tattctctgt acgtgagact gtaccagggc
 5101 gacgtgtgcg agctgagggc aagcgatctg actgaagcag agtccaacct ggccaagacc
 5161 acacatgtcc gcctgcccaa tgctaaacct gggcgaacct tcgtgatcat tatcaccttt
 5221 acagagatgg ggtctggata tcagatctac ttcagcaacc tggccaagtc caaaaaggga
 5281 caggacacta gttttaccct gactaccatc aagaattatg atgtgcggaa agtccagctg
 5341 tctagtgccg ggctggtgag atacgtcagc cctctgctgg tggacaaaat cgagaaggat
 5401 gaagtcgctc tgtgtggaga gtaagaattc ggtaccgagc tcaagcttgc ggccgcctat
 5461 agtgtcacct aaatcgtaac ccagctttct tgtacaaagt ggtcgggtgg catccctgtg
 5521 acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg
 5581 tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg
 5641 ggtggagggg ggtggtatgg agcaaggggc aagttgggat gacaacctgt agggcctgcg
 5701 gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg
 5761 cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat
 5821 gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg
 5881 ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc
 5941 tgggattaca ggcgtgaacc actgctccct tccctgtcct tctagcataa ccccttgggg
 6001 cctctaaacg ggtcttgagg ggttttttgt ctagaagaca atagcaggca tgctggggat
 6061 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
 6121 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
 6181 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
 6241 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
 6301 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
 6361 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
 6421 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
 6481 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
 6541 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
 6601 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
 6661 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
 6721 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
 6781 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
 6841 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
 6901 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca
 6961 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
 7021 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
 7081 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
 7141 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
 7201 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
 7261 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
 7321 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
 7381 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
 7441 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
 7501 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
 7561 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
 7621 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
 7681 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
 7741 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
 7801 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
 7861 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
 7921 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
 7981 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
 8041 tgtatcttat catgtctgtt catgaagccc cttgagcatc tgacttctgg ctaataaagg
 8101 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
 8161 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
 8221 tatgcccata tgtaactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
 8281 ttgctgaaag catgcggagg aaattctcct tgaagtttcc ctggtgttca aagtaaagga
 8341 gtttgcacca gacgcacctc tgttcactgg tccggcgtat taaaacacga tacattgtta
 8401 ttagtacatt tattaagcgc tagattctgt gcgttgttga tttacagaca attgttgtac
 8461 gtattttaat aattcattaa atttataatc tttagggtgg tatgttagag cgaaaatcaa
 8521 atgattttca gcgtctttat atctgaattt aaatattaaa tcctcaatag atttgtaaaa
 8581 taggtttcga ttagtttcaa acaagggttg tttttccgaa ccgatggctg gactatctaa
 8641 tggattttcg ctcaacgcca caaaacttgc caaatcttgt agcagcaatc tagctttgtc
 8701 gatattcgtt tgtgttttgt tttgtaataa aggttcgacg tcgttcaaaa tattatgcgc
 8761 ttttgtattt ctttcatcac tgtcgttagt gtacaattga ctcgacgtaa acacgttaaa
 8821 tagagcttgg acatatttaa catcgggcgt gttagcttta ttaggccgat tatcgtcgtc
 8881 gtcccaaccc tcgtcgttag aagttgcttc cgaagacgat tttgccatag ccacacgacg
 8941 cctattaatt gtgtcggcta acacgtccgc gatcaaattt gtagttgagc tttttggaat
 9001 tgcgatcgca taacttcgta tagcatacat tatacgaagt tataagctcg gaacgctgcg
 9061 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
 9121 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
 9181 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc tgacgagcat
 9241 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
 9301 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
 9361 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
 9421 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
 9481 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
 9541 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
 9601 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
 9661 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
 9721 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
 9781 agaaaaaaag gatctcaaga agatcctttg ttaccaatgc ttaatcagtg aggcacctat
 9841 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
 9901 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
 9961 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
10021 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
10081 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
10141 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
10201 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
10261 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
10321 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
10381 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
10441 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
10501 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
10561 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
10621 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
10681 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgtccgcgc gtt

//

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