pCS2+

價格:2500元

聯系方式:I47-825O-882O

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載體基本信息

出品公司: --
載體名稱: pCS2(+)
質粒類型: 克隆載體;哺乳動物表達載體;廣宿主載體;體外表達載體
高拷貝/低拷貝: 高拷貝
克隆方法: 限制性內切酶,多克隆位點
啟動子: simian CMV IE94、lac、T3、SP6
載體大小: 4095 bp
5' 測序引物及序列: SP6 fwd(MCS1)、T3 fwd(MCS2)
3' 測序引物及序列: EBV rev(MCS1)、SV40 polyA rev(MCS2)
載體標簽:
載體抗性: 氨芐青霉素
克隆菌株: DH10B
表達細胞: 原核細胞、哺乳動物、非洲爪蟾、鳥類、斑馬魚。
備注: pCS2(+)載體能夠在多種細胞中進行高水平的瞬表達。pCS2(+)載 體同時也能進行體外轉錄和翻譯。
產品目錄號: --
穩定性: 穩表達
組成型/誘導型: 組成型
病毒/非病毒: 非病毒

載體質粒圖譜和多克隆位點信息

pCS2(+)載體圖譜



pCS2(+) 多克隆位點

1

pCS2(+) 多克隆位點

2

載體簡介

pCS2+ contains a strong enhancer/promoter (simian CMV IE94) followed by a polylinker and the SV40 late polyadenlyation site. An SP6 promoter is present in the 5' untranslated region of the mRNA from the sCMV promoter, allowing in vitro RNA synthesis of sequences cloned into the polylinker. A T7 promoter in reverse orientation between the polylinker and the SV40 polyA site for probe synthesis, as well as a second polylinker after the SV40 polyA site to provide several possible sites to linearize the vector for SP6 RNA transcription. The vector backbone is from pBluescript II KS+ and includes the amp resistance gene and an f1 origin for producing single stranded DNA.

載體序列

LOCUS       Exported                4095 bp ds-DNA     circular SYN 29-JUL-2016
DEFINITION  Vector that allows high-level transient expression in vertebrate 
            cells as well as in vitro transcription/translation.
KEYWORDS    pCS2+
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Biofeng Lab.
COMMENT     This file is created by Vector NTI
            http://www.vpiqnf.icu/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pCS2(+)|
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        35..53
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    79..120
                     /note="MCS1"
     misc_feature    117..144
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    144..278
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    345..378
                     /note="MCS2"
     promoter        complement(391..409)
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    complement(432..434)
                     /note="ATG"
     protein_bind    454..470
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(478..508)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(832..1420)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1591..2451)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2452..2556)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      complement(2582..3037)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3111..4095
                     /note="CMV IE94 promoter"
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattca aggcctctcg agcctctaga
      121 actatagtga gtcgtattac gtagatccag acatgataag atacattgat gagtttggac
      181 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
      241 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      301 ttatgtttca ggttcagggg gaggtgtggg aggtttttta attcgcggcc gcggcgccaa
      361 tgcattgggc ccggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc
      421 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
      481 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      541 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
      601 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
      661 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      721 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
      781 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      841 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      901 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      961 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     1021 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     1081 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     1141 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     1201 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     1261 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     1321 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     1381 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1441 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     1501 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     1561 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     1621 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     1681 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     1741 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     1801 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     1861 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     1921 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     1981 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     2041 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     2101 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     2161 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     2221 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     2281 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     2341 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     2401 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     2461 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     2521 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     2581 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     2641 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     2701 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     2761 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     2821 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     2881 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     2941 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     3001 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
     3061 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagtc gaccatagcc
     3121 aattcaatat ggcgtatatg gactcatgcc aattcaatat ggtggatctg gacctgtgcc
     3181 aattcaatat ggcgtatatg gactcgtgcc aattcaatat ggtggatctg gaccccagcc
     3241 aattcaatat ggcggacttg gcaccatgcc aattcaatat ggcggacttg gcactgtgcc
     3301 aactggggag gggtctactt ggcacggtgc caagtttgag gaggggtctt ggccctgtgc
     3361 caagtccgcc atattgaatt ggcatggtgc caataatggc ggccatattg gctatatgcc
     3421 aggatcaata tataggcaat atccaatatg gccctatgcc aatatggcta ttggccaggt
     3481 tcaatactat gtattggccc tatgccatat agtattccat atatgggttt tcctattgac
     3541 gtagatagcc cctcccaatg ggcggtccca tataccatat atggggcttc ctaataccgc
     3601 ccatagccac tcccccattg acgtcaatgg tctctatata tggtctttcc tattgacgtc
     3661 atatgggcgg tcctattgac gtatatggcg cctcccccat tgacgtcaat tacggtaaat
     3721 ggcccgcctg gctcaatgcc cattgacgtc aataggacca cccaccattg acgtcaatgg
     3781 gatggctcat tgcccattca tatccgttct cacgccccct attgacgtca atgacggtaa
     3841 atggcccact tggcagtaca tcaatatcta ttaatagtaa cttggcaagt acattactat
     3901 tggaaggacg ccagggtaca ttggcagtac tcccattgac gtcaatggcg gtaaatggcc
     3961 cgcgatggct gccaagtaca tccccattga cgtcaatggg gaggggcaat gacgcaaatg
     4021 ggcgttccat tgacgtaaat gggcggtagg cgtgcctaat gggaggtcta tataagcaat
     4081 gctcgtttag ggaac
//

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