pBTB-5

價格:3000元

聯系方式:I47-825O-882O

買家導航

載體基本信息

出品人: Lynch,M.D. and Gill,R.T.
載體名稱: pBTB-5
質粒類型: 廣宿主載體;克隆載體;原核表達載體
高拷貝/低拷貝: --
克隆方法: 限制性內切酶,多克隆位點
啟動子: araBAD
載體大小: 3161 bp
5' 測序引物及序列: --
3' 測序引物及序列: --
載體標簽: --
載體抗性: 甲氧氨芐嘧啶
克隆菌株: DH5α 等
宿主細胞(系): 多個菌屬
備注: --
產品目錄號: --
組成型/誘導型: 誘導型

載體質粒圖譜和多克隆位點信息

pBTB-5載體圖譜



載體簡介

載體序列

LOCUS       DQ058733                3161 bp    DNA     circular SYN 08-MAY-2006
DEFINITION  Broad host range vector pBTB-5, complete sequence.
  AUTHORS   Lynch,M.D. and Gill,R.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2005) Chemical & Biological Engineering,
            University of Colorado, ECCH 111, Campus Box 424, Boulder, CO
            80309, USA
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Broad host range vector pBTB-5"
                     /mol_type="other DNA"
                     /db_xref="taxon:334965"
     regulatory      60..96
                     /regulatory_class="terminator"
                     /note="SoxR terminator"
     gene            872..1534
                     /gene="rep"
                     /note="rep gene from pBBR1, GenBank Accession Number
                     X66730"
     CDS             872..1534
                     /gene="rep"
                     /note="required for replication"
                     /codon_start=1
                     /transl_table=11
                     /product="Rep"
                     /protein_id="AAY99729.1"
                     /db_xref="GI:69048360"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
                     HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
                     VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
                     GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
                     RR"
     gene            1631..1867
                     /gene="dhfrIIb"
                     /note="dhfrIIb gene from R388"
     CDS             1631..1867
                     /gene="dhfrIIb"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="AAY99730.1"
                     /db_xref="GI:69048361"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVG
                     WYCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     regulatory      1884..1915
                     /regulatory_class="terminator"
                     /note="TonB terminator"
     gene            complement(1943..2872)
                     /gene="araC"
     CDS             complement(1943..2872)
                     /gene="araC"
                     /note="regulator"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC"
                     /protein_id="AAY99731.1"
                     /db_xref="GI:69048362"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS"
     regulatory      2880..3147
                     /regulatory_class="promoter"
                     /note="pBAD promoter"
ORIGIN      
        1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg
      121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca
      181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc
      241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag
      301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc
      361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc
      421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg
      481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt
      541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg
      601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg
      661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac
      721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg
      781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa
      841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat
      901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg
      961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca
     1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg
     1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt
     1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg
     1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca
     1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta
     1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat
     1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg
     1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga
     1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca
     1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
     1621 ggaagctaaa atgggtcaaa gtagcgatga agccaacgct cccgttgcag ggcagtttgc
     1681 gcttcccctg agtgccacct ttggcttagg ggatcgcgta cgcaagaaat ctggtgccgc
     1741 ttggcagggt caagtcgtcg gttggtattg cacaaaactc actcctgaag gctatgcggt
     1801 cgagtccgaa tcccacccag gctcagtgca aatttatcct gtggctgcac ttgaacgtgt
     1861 ggcctaagaa tggatccccc tcaagtcaaa agcctccggt cggaggcttt tgactttctg
     1921 ctatggaggt caggtatgat ttttatgaca acttgacggc tacatcattc actttttctt
     1981 cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga
     2041 aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg
     2101 tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa
     2161 tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg
     2221 cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct
     2281 cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca
     2341 gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc
     2401 cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc
     2461 gaaagaaccc cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc
     2521 gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt
     2581 gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc
     2641 cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc
     2701 ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg
     2761 ccaccagatg ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca
     2821 tacttttcat actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt
     2881 gccgtcactg cgtcttttac tggctcttct cgctaaccaa accggtaacc ccgcttatta
     2941 aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct
     3001 ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca
     3061 tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta
     3121 ctgtttctcc atacccgttt ttttgggcta gagaattcgt c
//

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