pBTB-3

價格:3000元

聯系方式:I47-825O-882O

買家導航

載體基本信息

出品人: Lynch,M.D. and Gill,R.T.
載體名稱: pBTB-3
質粒類型: 廣宿主載體;克隆載體;原核表達載體
高拷貝/低拷貝: --
克隆方法: 限制性內切酶,多克隆位點
啟動子: araBAD
載體大小: 3586 bp
5' 測序引物及序列: --
3' 測序引物及序列: --
載體標簽: --
載體抗性: 氯霉素
克隆菌株: DH5α 等
宿主細胞(系): 多個菌屬
備注: --
產品目錄號: --
組成型/誘導型: 誘導型

載體質粒圖譜和多克隆位點信息

pBTB-3載體圖譜



載體簡介

載體序列

LOCUS       DQ058731                3586 bp    DNA     circular SYN 08-MAY-2006
DEFINITION  Broad host range vector pBTB-3, complete sequence.
ACCESSION   DQ058731
  AUTHORS   Lynch,M.D. and Gill,R.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2005) Chemical & Biological Engineering,
            University of Colorado, ECCH 111, Campus Box 424, Boulder, CO
            80309, USA
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Broad host range vector pBTB-3"
                     /mol_type="other DNA"
                     /db_xref="taxon:334963"
     regulatory      60..96
                     /regulatory_class="terminator"
                     /note="SoxR terminator"
     gene            872..1534
                     /gene="rep"
                     /note="rep gene from pBBR1, GenBank Accession Number
                     X66730"
     CDS             872..1534
                     /gene="rep"
                     /note="required for replication"
                     /codon_start=1
                     /transl_table=11
                     /product="Rep"
                     /protein_id="AAY99723.1"
                     /db_xref="GI:69048329"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
                     HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
                     VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
                     GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
                     RR"
     gene            1631..2290
                     /gene="cat"
     CDS             1631..2290
                     /gene="cat"
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyl transferase"
                     /protein_id="AAY99724.1"
                     /db_xref="GI:69048330"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     regulatory      2309..2340
                     /regulatory_class="terminator"
                     /note="TonB terminator"
     gene            complement(2368..3297)
                     /gene="araC"
     CDS             complement(2368..3297)
                     /gene="araC"
                     /note="regulator"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC"
                     /protein_id="AAY99725.1"
                     /db_xref="GI:69048331"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS"
     regulatory      3305..3572
                     /regulatory_class="promoter"
                     /note="pBAD promoter"
ORIGIN      
        1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg
      121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca
      181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc
      241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag
      301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc
      361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc
      421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg
      481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt
      541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg
      601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg
      661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac
      721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg
      781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa
      841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat
      901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg
      961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca
     1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg
     1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt
     1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg
     1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca
     1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta
     1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat
     1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg
     1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga
     1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca
     1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
     1621 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
     1681 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
     1741 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
     1801 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
     1861 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
     1921 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
     1981 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
     2041 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
     2101 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
     2161 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     2221 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
     2281 cggggcgtaa acgcgtggat ccccctcaag tcaaaagcct ccggtcggag gcttttgact
     2341 ttctgctatg gaggtcaggt atgattttta tgacaacttg acggctacat cattcacttt
     2401 ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg
     2461 cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg
     2521 ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac
     2581 gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg
     2641 ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca
     2701 agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg
     2761 ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc
     2821 ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc
     2881 cgggcgaaag aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta
     2941 ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc
     3001 gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc
     3061 tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt
     3121 cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa
     3181 acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc
     3241 agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag
     3301 acattgccgt cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct
     3361 tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag
     3421 tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta
     3481 tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact
     3541 ctctactgtt tctccatacc cgtttttttg ggctagagaa ttcgtc
//

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