pBMTB-4

價格:2500元

聯系方式:I47-825O-882O

買家導航

載體基本信息

出品人: Lynch,M.D. and Gill,R.T.
載體名稱: pBMTB-4
質粒類型: 廣宿主載體;克隆載體;原核表達載體
高拷貝/低拷貝: --
克隆方法: 限制性內切酶,多克隆位點
啟動子: araBAD
載體大小: 5363 bp
5' 測序引物及序列: --
3' 測序引物及序列: --
載體標簽: --
載體抗性: 四環素
克隆菌株: DH5α 等
宿主細胞(系): 多個菌屬
備注: --
產品目錄號: --
組成型/誘導型: 誘導型

載體質粒圖譜和多克隆位點信息

pBMTB-4載體圖譜



載體簡介

載體序列

LOCUS       DQ058727                5363 bp    DNA     circular SYN 08-MAY-2006
DEFINITION  Broad host range vector pBMTB-4, complete sequence.
ACCESSION   DQ058727
  AUTHORS   Lynch,M.D. and Gill,R.T.
  TITLE     Broad host range vectors for stable genomic library construction
  JOURNAL   Submitted (12-MAY-2005) Chemical & Biological Engineering,
            University of Colorado, ECCH 111, Campus Box 424, Boulder, CO
            80309, USA
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Broad host range vector pBMTB-4"
                     /mol_type="other DNA"
                     /db_xref="taxon:334959"
     regulatory      60..96
                     /regulatory_class="terminator"
                     /note="SoxR terminator"
     gene            complement(104..1123)
                     /gene="mob"
                     /note="mob gene from pBBR1MCS, GenBank Accession Number
                     U02374"
     CDS             complement(104..1123)
                     /gene="mob"
                     /note="required for mobilization"
                     /codon_start=1
                     /transl_table=11
                     /product="Mob"
                     /protein_id="AAY99709.1"
                     /db_xref="GI:69048280"
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH
                     WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW
                     LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA
                     AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG
                     ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL
                     DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSK
                     KPLLS"
     gene            2117..2779
                     /gene="rep"
                     /note="rep gene from pBBR1, GenBank Accession Number
                     X66730"
     CDS             2117..2779
                     /gene="rep"
                     /note="required for replication"
                     /codon_start=1
                     /transl_table=11
                     /product="Rep"
                     /protein_id="AAY99710.1"
                     /db_xref="GI:69048281"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
                     HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
                     VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
                     GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
                     RR"
     gene            2876..4066
                     /gene="tetA"
                     /note="tetracycline resistance gene from pBR322"
     CDS             2876..4066
                     /gene="tetA"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline/H+ antiporter"
                     /protein_id="AAY99711.1"
                     /db_xref="GI:69048282"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
                     YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
                     RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
                     APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
                     FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
                     KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
                     QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
                     ATST"
     regulatory      4086..4117
                     /regulatory_class="terminator"
                     /note="TonB terminator"
     gene            complement(4145..5074)
                     /gene="araC"
     CDS             complement(4145..5074)
                     /gene="araC"
                     /note="regulator"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC"
                     /protein_id="AAY99712.1"
                     /db_xref="GI:69048283"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS"
     regulatory      5082..5349
                     /regulatory_class="promoter"
                     /note="pBAD promoter"
ORIGIN      
        1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgctcatgat aataatggtt
      121 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg cccgcgttcc tgctggcgct
      181 gggcctgttt ctggcgctgg acttcccgct gttccgtcag cagcttttcg cccacggcct
      241 tgatgatcgc ggcggccttg gcctgcatat cccgattcaa cggccccagg gcgtccagaa
      301 cgggcttcag gcgctcccga aggtctcggg ccgtctcttg ggcttgatcg gccttcttgc
      361 gcatctcacg cgctcctgcg gcggcctgta gggcaggctc atacccctgc cgaaccgctt
      421 ttgtcagccg gtcggccacg gcttccggcg tctcaacgcg ctttgagatt cccagctttt
      481 cggccaatcc ctgcggtgca taggcgcgtg gctcgaccgc ttgcgggctg atggtgacgt
      541 ggcccactgg tggccgctcc agggcctcgt agaacgcctg aatgcgcgtg tgacgtgcct
      601 tgctgccctc gatgccccgt tgcagcccta gatcggccac agcggccgca aacgtggtct
      661 ggtcgcgggt catctgcgct ttgttgccga tgaactcctt ggccgacagc ctgccgtcct
      721 gcgtcagcgg caccacgaac gcggtcatgt gcgggctggt ttcgtcacgg tggatgctgg
      781 ccgtcacgat gcgatccgcc ccgtacttgt ccgccagcca cttgtgcgcc ttctcgaaga
      841 acgccgcctg ctgttcttgg ctggccgact tccaccattc cgggctggcc gtcatgacgt
      901 actcgaccgc caacacagcg tccttgcgcc gcttctctgg cagcaactcg cgcagtcggc
      961 ccatcgcttc atcggtgctg ctggccgccc agtgctcgtt ctctggcgtc ctgctggcgt
     1021 cagcgttggg cgtctcgcgc tcgcggtagg cgtgcttgag actggccgcc acgttgccca
     1081 ttttcgccag cttcttgcat cgcatgatcg cgtatgccgc catgcctgcc cctccctttt
     1141 ggtgtccaac cggctcgacg ggggcagcgc aaggcggtgc ctccggcggg ccactcaatg
     1201 cttgagtata ctcactagac tttgcttcgc aaagtcgtga ccgcctacgg cggctgcggc
     1261 gccctacggg cttgctctcc gggcttcgcc ctgcgcggtc gctgcgctcc cttgccagcc
     1321 cgtggatatg tggacgatgg ccgcgagcgg ccaccggctg gctcgcttcg ctcggcccgt
     1381 ggacaaccct gctggacaag ctgatggaca ggctgcgcct gcccacgagc ttgaccacag
     1441 ggattgccca ccggctaccc agccttcgac cacataccca ccggctccaa ctgcgcggcc
     1501 tgcggccttg ccccatcaat ttttttaatt ttctctgggg aaaagcctcc ggcctgcggc
     1561 ctgcgcgctt cgcttgccgg ttggacacca agtggaaggc gggtcaaggc tcgcgcagcg
     1621 accgcgcagc ggcttggcct tgacgcgcct ggaacgaccc aagcctatgc gagtgggggc
     1681 agtcgaaggc gaagcccgcc cgcctgcccc ccgagcctca cggcggcgag tgcgggggtt
     1741 ccaagggggc agcgccacct tgggcaaggc cgaaggccgc gcagtcgatc aacaagcccc
     1801 ggaggggcca ctttttgccg gagggggagc cgcgccgaag gcgtggggga accccgcagg
     1861 ggtgcccttc tttgggcacc aaagaactag atatagggcg aaatgcgaaa gacttaaaaa
     1921 tcaacaactt aaaaaagggg ggtacgcaac agctcattgc ggcacccccc gcaatagctc
     1981 attgcgtagg ttaaagaaaa tctgtaattg actgccactt ttacgcaacg cataattgtt
     2041 gtcgcgctgc cgaaaagttg cagctgattg cgcatggtgc cgcaaccgtg cggcacccta
     2101 ccgcatggag ataagcatgg ccacgcagtc cagagaaatc ggcattcaag ccaagaacaa
     2161 gcccggtcac tgggtgcaaa cggaacgcaa agcgcatgag gcgtgggccg ggcttattgc
     2221 gaggaaaccc acggcggcaa tgctgctgca tcacctcgtg gcgcagatgg gccaccagaa
     2281 cgccgtggtg gtcagccaga agacactttc caagctcatc ggacgttctt tgcggacggt
     2341 ccaatacgca gtcaaggact tggtggccga gcgctggatc tccgtcgtga agctcaacgg
     2401 ccccggcacc gtgtcggcct acgtggtcaa tgaccgcgtg gcgtggggcc agccccgcga
     2461 ccagttgcgc ctgtcggtgt tcagtgccgc cgtggtggtt gatcacgacg accaggacga
     2521 atcgctgttg gggcatggcg acctgcgccg catcccgacc ctgtatccgg gcgagcagca
     2581 actaccgacc ggccccggcg aggagccgcc cagccagccc ggcattccgg gcatggaacc
     2641 agacctgcca gccttgaccg aaacggagga atgggaacgg cgcgggcagc agcgcctgcc
     2701 gatgcccgat gagccgtgtt ttctggacga tggcgagccg ttggagccgc cgacacgggt
     2761 cacgctgccg cgccggtagt acgtaagagg ttccaacttt caccataatg aaataagatc
     2821 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgaa
     2881 atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg
     2941 cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg acagcatcgc
     3001 cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat gcgcacccgt
     3061 tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt cgctacttgg
     3121 agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggatcc tctacgccgg
     3181 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
     3241 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
     3301 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
     3361 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
     3421 ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc
     3481 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat
     3541 gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg
     3601 ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc
     3661 tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc
     3721 cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat
     3781 ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc
     3841 catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc
     3901 tcttaccagc ctaacttcga tcactggacc gctgatcgtc acggcgattt atgccgcctc
     3961 ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct
     4021 ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg aagggatccc
     4081 cctcaagtca aaagcctccg gtcggaggct tttgactttc tgctatggag gtcaggtatg
     4141 atttttatga caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact
     4201 cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa
     4261 aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg
     4321 cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga
     4381 aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa
     4441 aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca
     4501 tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca
     4561 gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc
     4621 caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg
     4681 caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc
     4741 actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg
     4801 ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc
     4861 cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa
     4921 aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa
     4981 acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc
     5041 cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt
     5101 actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa
     5161 gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag
     5221 tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa
     5281 gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt
     5341 ttttttgggc tagagaattc gtc
//

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