pAdDeltaF6

價格:1200元

聯系方式:I47-825O-882O

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載體基本信息

質粒名稱: pAdDeltaF6
出品公司: 愛迪基因
目錄編號: 112867
存儲實驗室: James M. Wilson

載體骨架基本信息
載體名稱: --
載體出品公司: --
載體抗性: 氨芐青霉素
空載體大小: --
完整質粒大小: --
載體修飾: --
載體類型: AAV
篩選標記: --
高拷貝/低拷貝: 未知
生長條件: 37 ℃

插入基因信息
插入基因名稱: E4, E2a and VA
物種來源: 腺病毒
插入大小: --
基因突變: --
GenBank編號 --
啟動子 --
標簽/融合蛋白: --
克隆方法: --
5' 克隆位點: --
3' 克隆位點: --
5' 測序引物 --
3' 測序引物 --

文獻引用方法
材料和方法部分: pAdDeltaF6 was a gift from James M. Wilson (Addgene plasmid # 112867 ; http://n2t.net/addgene:112867 ; RRID:Addgene_112867)

載體質粒圖譜和多克隆位點信息

pAdDeltaF6

載體簡介

載體序列

LOCUS       pAdDeltaF6              15420 bp ds-DNA     circular SYN 17-MAY-2019
DEFINITION  AAV helper plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    pAdDeltaF6
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15420)
  TITLE     AAV packaging plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 17, 2019 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15420
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(46..62)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(46..62)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(99..119)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(99..117)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(138..154)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(138..154)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(151..173)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    162..178
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(186..216)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    231..252
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(369..386)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(540..1128)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(620..639)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1299..2159)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1922..1941
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2160..2264)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2332..2350
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2377..2405
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 tcgacggtat cgataagctt gatatcgaat tcctgcagcc cgggggatcc actagttcta
       61 gagcggccgc caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg
      121 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
      181 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
      241 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
      301 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
      361 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      421 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
      481 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      541 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      601 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      661 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      721 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      781 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
      841 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
      901 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
      961 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1021 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1081 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     1141 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     1201 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     1261 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     1321 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     1381 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     1441 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     1501 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     1561 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     1621 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     1681 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     1741 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     1801 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     1861 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     1921 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     1981 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     2041 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     2101 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     2161 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     2221 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     2281 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     2341 atcacgaggc cctttcgtct tcaagaattc tcatgtttga cagcttatca tcgataagct
     2401 ttaatgcggt agtttatcac agttaaattg ctaacgcagt caggcaccgt gtatgaaatc
     2461 taacaatgcg ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag gcataggctt
     2521 ggttatgccg gtactgccgg gcctcttgcg ggatatcgtc cattccgaca gcatcgccag
     2581 tcactatggc gtgctgctag cgctatatgc gttgatgcaa tttctatgcg cacccgttct
     2641 cggagcactg tccgaccgct ttggccgccg cccagtcctg ctcgcttcgc tacttggagc
     2701 cactatcgac tacgcgatca tggcgaccac acccgtcctg tggatccggc gcacaccaaa
     2761 aacgtcactt ttgccacatc cgtcgcttac atgtgttccg ccacacttgc aacatcacac
     2821 ttccgccaca ctactacgtc acccgccccg ttcccacgcc ccgcgccacg tcacaaactc
     2881 caccccctca ttatcatatt ggcttcaatc caaaataatc atcaataata taccttattt
     2941 tggattgaag ccaatatgat aatgaggggg tggagtttgt gacgtggcgc ggggcgtggg
     3001 aacggggcgg gtgacgtagg ttttagggcg gagtaacttg tatgtgttgg gaattgtagt
     3061 tttcttaaaa tgggaagtga cgtaacgtgg gaaaacggaa gtgacgattt gaggaagttg
     3121 tgggtttttt ggctttcgtt tctgggcgta ggttcgcgtg cggttttctg ggtgtttttt
     3181 gtggacttta accgttacgt cattttttag tcctatatat actcgctctg cacttggccc
     3241 ttttttacac tgtgactgat tgagctggtg ccgtgtcgag tggtgttttt ttaataggtt
     3301 ttctttttta ctggtaaggc tgactgttat ggctgccgct gtggaagcgc tgtatgttgt
     3361 tctggagcgg gagggtgcta ttttgcctag gcaggagggt ttttcaggtg tttatgtgtt
     3421 tttctctcct attaattttg ttatacctcc tatgggggct gtaatgttgt ctctacgcct
     3481 gcgggtatgt attcccccgg gctatttcgg tcgcttttta gcactgaccg atgtgaatca
     3541 acctgatgtg tttaccgagt cttacattat gactccggac atgaccgagg agctgtcggt
     3601 ggtgcttttt aatcacggtg accagttttt ttacggtcac gccggcatgg ccgtagtccg
     3661 tcttatgctt ataagggttg tttttcctgt tgtaagacag gcttctaatg tttaaatgtt
     3721 tttttgttat tttattttgt gtttatgcag aaacccgcag acatgtttga gagaaaaatg
     3781 gtgtcttttt ctgtggtggt tccggagctt acctgccttt atctgcatga gcatgactac
     3841 gatgtgcttt cttttttgcg cgaggctttg cctgattttt tgagcagcac cttgcatttt
     3901 atatcgccgc ccatgcaaca agcttacatc ggggctacgc tggttagcat agctccgagt
     3961 atgcgtgtca taatcagtgt gggttctttt gtcatggttc ctggcgggga agtggccgcg
     4021 ctggtccgtg cagacctgca cgattatgtt cagctggccc tgcgaaggga cctacgggat
     4081 cgcggtattt ttgttaatgt tccgcttttg aatcttatac aggtctgtga ggaacctgaa
     4141 tttttgcaat catgattcgc tgcttgaggc tgaaggtgga gggcgctctg gagcagattt
     4201 ttacaatggc cggacttaat attcgggatt tgcttagaga tatattgaga aggtggcgag
     4261 atgagaatta tttgggcatg gttgaaggtg ctggaatgtt tatagaggag attcaccctg
     4321 aagggtttag cctttacgtc cacttggacg tgagggccgt ttgccttttg gaagccattg
     4381 tgcaacatct tacaaatgcc attatctgtt ctttggctgt agagtttgac cacgccaccg
     4441 gaggggagcg cgttcactta atagatcttc attttgaggt tttggataat cttttggaat
     4501 aaaaaaaaaa acatggttct tccagctctt cccgctcctc ccgtgtgtga ctcgcagaac
     4561 gaatgtgtag gttggctggg tgtggcttat tctgcggtgg tggatgttat cagggcagcg
     4621 gcgcatgaag gagtttacat agaacccgaa gccagggggc gcctggatgc tttgagagag
     4681 tggatatact acaactacta cacagagcga tctaagcggc gagaccggag acgcagatct
     4741 gtttgtcacg cccgcacctg gttttgcttc aggaaatatg actacgtccg gcgttccatt
     4801 tggcatgaca ctacgaccaa cacgatctcg gttgtctcgg cgcactccgt acagtaggga
     4861 tcgtctacct ccttttgaga cagaaacccg cgctaccata ctggaggatc atccgctgct
     4921 gcccgaatgt aacactttga caatgcacaa cgtgagttac gtgcgaggtc ttccctgcag
     4981 tgtgggattt acgctgattc aggaatgggt tgttccctgg gatatggttc taacgcggga
     5041 ggagcttgta atcctgagga agtgtatgca cgtgtgcctg tgttgtgcca acattgatat
     5101 catgacgagc atgatgatcc atggttacga gtcctgggct ctccactgtc attgttccag
     5161 tcccggttcc ctgcagtgta tagccggcgg gcaggttttg gccagctggt ttaggatggt
     5221 ggtggatggc gccatgttta atcagaggtt tatatggtac cgggaggtgg tgaattacaa
     5281 catgccaaaa gaggtaatgt ttatgtccag cgtgtttatg aggggtcgcc acttaatcta
     5341 cctgcgcttg tggtatgatg gccacgtggg ttctgtggtc cccgccatga gctttggata
     5401 cagcgccttg cactgtggga ttttgaacaa tattgtggtg ctgtgctgca gttactgtgc
     5461 tgatttaagt gagatcaggg tgcgctgctg tgcccggagg acaaggcgcc ttatgctgcg
     5521 ggcggtgcga atcatcgctg aggagaccac tgccatgttg tattcctgca ggacggagcg
     5581 gcggcggcag cagtttattc gcgcgctgct gcagcaccac cgccctatcc tgatgcacga
     5641 ttatgactct acccccatgt aggcgtggac ttctccttcg ccgcccgtta agcaaccgca
     5701 agttggacag cagcctgtgg ctcagcagct ggacagcgac atgaacttaa gtgagctgcc
     5761 cggggagttt attaatatca ctgatgagcg tttggctcga caggaaaccg tgtggaatat
     5821 aacacctaag aatatgtctg ttacccatga tatgatgctt tttaaggcca gccggggaga
     5881 aaggactgtg tactctgtgt gttgggaggg aggtggcagg ttgaatacta gggttctgtg
     5941 agtttgatta aggtacggtg atctgtataa gctatgtggt ggtggggcta tactactgaa
     6001 tgaaaaatga cttgaaattt tctgcaattg aaaaataaac acgttgaaac ataacacaaa
     6061 cgattcttta ttcttgggca atgtatgaaa aagtgtaaga ggatgtggca aatatttcat
     6121 taatgtagtt gtggccagac cagtcccatg aaaatgacat agagtatgca cttggagttg
     6181 tgtctcctgt ttcctgtgta ccgtttagtg taatggttag tgttacaggt ttagttttgt
     6241 ctccgtttaa gtaaacttga ctgacaatgt tacttttggc agttttaccg tgagattttg
     6301 gataagctga taggttaggc ataaatccaa cagcgtttgt ataggctgtg ccttcagtaa
     6361 gatctccatt tctaaagttc caatattctg ggtccaggaa ggaattgttt agtagcactc
     6421 cattttcgtc aaatcttata ataagatgag cactttgaac tgttccagat attggagcca
     6481 aactgccttt aacagccaaa actgaaactg tagcaagtat ttgactgcca cattttgtta
     6541 agaccaaagt gagtttagca tctttctctg catttagtct acagttagga gatggagctg
     6601 gtgtggtcca caaagttagc ttatcattat ttttgtttcc tactgtaatg gcacctgtgc
     6661 tgtcaaaact aaggccagtt cctagtttag gaaccatagc cttgtttgaa tcaaattcta
     6721 ggccatggcc aatttttgtt ttgaggggat ttgtgtttgg tgcattaggt gaaccaaatt
     6781 caagcccatc tcctgcatta atggctatgg ctgtagcgtc aaacatcaac cccttggcag
     6841 tgcttaggtt aacctcaagc tttttggaat tgtttgaagc tgtaaacaag taaaggcctt
     6901 tgttgtagtt aatatccaag ttgtgggctg agtttataaa aagagggccc tgtcctagtc
     6961 ttagatttag ttggttttga gcatcaaacg gataactaac atcaagtata aggcgtctgt
     7021 tttgagaatc aatccttagt cctcctgcta cattaagttg catattgcct tgtgaatcaa
     7081 aacccaaggc tccagtaact ttagtttgca aggaagtatt attaatagtc acacctggac
     7141 cagttgctac ggtcaaagtg tttaggtcgt ctgttacatg caaaggagcc ccgtacttta
     7201 gtcctagttt tccattttgt gtataaatgg gctctttcaa gtcaatgccc aagctaccag
     7261 tggcagtagt tagagggggt gaggcagtga tagtaagggt actgctatcg gtggtggtga
     7321 gggggcctga tgtttgcagg gctagctttc cttctgacac tgtgaggggt ccttgggtgg
     7381 caatgctaag tttggagtcg tgcacggtta gcggggcctg tgattgcatg gtgagtgtgt
     7441 tgcccgcgac cattagaggt gcggcggcag ccacagttag ggcttctgag gtaactgtga
     7501 ggggtgcaga tatttccagg tttatgtttg acttggtttt tttgagaggt gggctcacag
     7561 tggttacatt ttgggaggta aggttgccgg cctcgtccag agagaggccg ttgcccattt
     7621 tgagcgcaag catgccattg gaggtaacta gaggttcgga taggcgcaaa gagagtaccc
     7681 cagggggact ctcttgaaac ccattggggg atacaaaggg aggagtaaga aaaggcacag
     7741 ttggaggacc ggtttccgtg tcatatggat acacggggtt gaaggtatct tcagacggtc
     7801 ttgcgcgctt catctgcaac aacatgaaga tagtgggtgc ggatggacag gaacaggagg
     7861 aaactgacat tccatttaga ttgtggagaa agtttgcagc caggaggaag ctgcaatacc
     7921 agagctggga ggagggcaag gaggtgctgc tgaataaact ggacagaaat ttgctaactg
     7981 attttaagta agtgatgctt tattattttt ttttattagt taaagggaat aagatccccg
     8041 ggtactctag ttaattaact agaggatctt gatgtaatcc agggttagga cagttgcaaa
     8101 tcacagtgag aacacagggt cccctgtccc gctcaactag cagggggcgc tgggtaaact
     8161 cccgaatcag gctacgggca agctctccct gggcggtaag ccggacgccg tgcgccgggc
     8221 cctcgatatg atcctcgggc aattcaaagt agcaaaactc accggagtcg cgggcaaagc
     8281 acttgtggcg gcgacagtgg accaggtgtt tcaggcgcag ttgctctgcc tctccactta
     8341 acattcagtc gtagccgtcc gccgagtcct ttaccgcgtc aaagttagga ataaattgat
     8401 ccggatagtg gccgggaggt cccgagaagg ggttaaagta gaccgatggc acaaactcct
     8461 caataaattg cagagttcca atgcctccag agcgcggctc agaggacgag gtctgcagag
     8521 ttaggattgc ctgacgaggc gtgaatgaag agcggccggc gccgccgatc tgaaatgtcc
     8581 cgtccggacg gagaccaagc gaggagctca ccgactcgtc gttgagctga atacctcgcc
     8641 ctctgattgt caggtgagtt ataccctgcc cgggcgaccg caccctgtga cgaaagccgc
     8701 ccgcaagctg cgcccctgag ttagtcatct gaacttcggc ctgggcgtct ctgggaagta
     8761 ccacagtggt gggagcggga ctttcctggt acaccagggc agcgggccaa ctacggggat
     8821 taaggttatt acgaggtgtg gtggtaatag ccgcctgttc caggagaatt cggtttcggt
     8881 gggcgcgtat tccgttgacc cgggatatca tgtggggtcc cgcgctcatg tagtttattc
     8941 gggttgagta gtcttgggca gctccagccg caagtcccat ttgtggctgg taactccaca
     9001 tgtagggcgt gggaatttcc ttgctcataa tggcgctgac aacaggtgct ggcgccgggt
     9061 gtggccgctg gagatgacgt agttttcgcg cttaaatttg agaaagggcg cgaaactagt
     9121 ccttaagagt cagcgcgcag tatttactga agagagcctc cgcgtcttcc agcgtgcgcc
     9181 gaagctgatc ttcgcttttg tgatacaggc agctgcgggt gagggatcgc agagacctgt
     9241 tttttatttt cagctcttgt tcttggcccc tgctctgttg aaatatagca tacagagtgg
     9301 gaaaaatcct gtttctaagc tcgcgggtcg atacgggttc gttgggcgcc agacgcagcg
     9361 ctcctcctcc tgctgctgcc gccgctgtgg atttcttggg ctttgtcaga gtcttgctat
     9421 ccggtcgcct ttgcttctgt gtggccgctg ctgttgctgc cgctgccgcc ggtgcagtat
     9481 gggctgtaga gatgacggta gtaatgcagg atgttacggg ggaaggccac gccgtgatgg
     9541 tagagaagaa agcggcgggc gaaggagatg ttgcccccac agtcttgcaa gcaagcaact
     9601 atggcgttct tgtgcccgcg ccatgagcgg tagccttggc gctgttgttg ctcttgggct
     9661 aacggcggcg gctgcttgga cttaccggcc ctggttccag tggtgtccca tctacggttg
     9721 ggtcggcgaa cgggcagtgc cggcggcgcc tgaggagcgg aggttgtagc catgctggaa
     9781 ccggttgccg atttctgggg cgccggcgag gggaatgcga ccgagggtga cggtgtttcg
     9841 tctgacacct cttcgacctc ggaagcttcc tcgtctaggc tctcccagtc ttccatcatg
     9901 tcctcctcct cctcgtccaa aacctcctct gcctgactgt cccagtattc ctcctcgtcc
     9961 gtgggtggcg gcggcagctg cagcttcttt ttgggtgcca tcctgggaag caagggcccg
    10021 cggctgctgc tgatagggct gcggcggcgg ggggattggg ttgagctcct cgccggactg
    10081 ggggtccaag taaacccccc gtccctttcg tagcagaaac tcttggcggg ctttgttgat
    10141 ggcttgcaat tggccaagaa tgtggccctg ggtaatgacg caggcggtaa gctccgcatt
    10201 aggcgggcgg gattggtctt cgtagaacct aatctcgtgg gcgtggtagt cctcaggtac
    10261 aaatttgcga aggtaagccg acgtccacag ccccggagtg agtttcaacc ccggagccgc
    10321 ggacttttcg tcaggcgagg gaccctgcag ctcaaaggta ccgataattt gactttcgtt
    10381 aagcagctgc gaattgcaaa ccagggagcg gtgcggggtg cataggttgc agcgacagtg
    10441 acactccagt agaccgtcac cgctcacgtc ttccattatg tcagagtggt aggcaaggta
    10501 gttggctagc tgcagaaggt agcagtggcc ccaaagcggc ggagggcatt cgcggtactt
    10561 aatgggcaca aagtcgctag gaagtgcaca gcaggtggcg ggcaagattc ctgagcgctc
    10621 taggataaag ttcctaaagt tctgcaacat gctttgactg gtgaagtctg gcagaccctg
    10681 ttgcagggtt ttaagcaggc gttcggggaa aatgatgtcc gccaggtgcg cggccacgga
    10741 gcgctcgttg aaggccgtcc ataggtcctt caagttttgc tttagcagtt tctgcagctc
    10801 cttgaggttg cactcctcca agcactgctg ccaaacgccc atggccgtct gccaggtgta
    10861 gcatagaaat aagtaaacgc agtcgcggac gtagtcgcgg cgcgcctcgc ccttgagcgt
    10921 ggaatgaagc acgttttgcc caaggcggtt ttcgtgcaaa attccaaggt aggagaccag
    10981 gttgcagagc tccacgttgg agatcttgca ggcctggcgt acgtagccct gtcgaaaggt
    11041 gtagtgcaat gtttcctcta gcttgcgctg catctccggg tcagcaaaga accgctgcat
    11101 gcactcaagc tccacggtaa cgagcactgc ggccatcatt agtttgcgtc gctcctccaa
    11161 gtcggcaggc tcgcgcgttt gaagccagcg cgctagctgc tcgtcgccaa ctgcgggtag
    11221 gccctcctct gtttgttctt gcaaatttgc atccctctcc aggggctgcg cacggcgcac
    11281 gatcagctca ctcatgactg tgctcatgac cttggggggt aggttaagtg ccgggtaggc
    11341 aaagtgggtg acctcgatgc tgcgttttag tacggctagg cgcgcgttgt caccctcgag
    11401 ttccaccaac actccagagt gactttcatt ttcgctgttt tcctgttgca gagcgtttgc
    11461 cgcgcgcttc tcgtcgcgtc caagaccctc aaagattttt ggcacttcgt tgagcgaggc
    11521 gatatcaggt atgacagcgc cctgccgcaa ggccagctgc ttgtccgctc ggctgcggtt
    11581 ggcacggcag gataggggta tcttgcagtt ttggaaaaag atgtgatagg tggcaagcac
    11641 ctctggcacg gcaaatacgg ggtagaagtt gaggcgcggg ttgggctcgc atgtgccgtt
    11701 ttcttggcgt ttggggggta cgcgcggtga gaataggtgg cgttcgtagg caaggctgac
    11761 atccgctatg gcgaggggca catcgctgcg ctcttgcaac gcgtcgcaga taatggcgca
    11821 ctggcgctgc agatgcttca acagcacgtc gtctcccaca tctaggtagt cgccatgcct
    11881 ttcgtccccc cgcccgactt gttcctcgtt tgcctctgcg ttgtcctggt cttgcttttt
    11941 atcctctgtt ggtactgagc ggtcctcgtc gtcttcgctt acaaaacctg ggtcctgctc
    12001 gataatcact tcctcctcct caagcggggg tgcctcgacg gggaaggtgg taggcgcgtt
    12061 ggcggcatcg gtggaggcgg tggtggcgaa ctcagagggg gcggttaggc tgtccttctt
    12121 ctcgactgac tccatgatct ttttctgcct ataggagaag gaaatggcca gtcgggaaga
    12181 ggagcagcgc gaaaccaccc ccgagcgcgg acgcggtgcg gcgcgacgtc ccccaaccat
    12241 ggaggacgtg tcgtccccgt ccccgtcgcc gccgcctccc cgggcgcccc caaaaaagcg
    12301 gatgaggcgg cgtatcgagt ccgaggacga ggaagactca tcacaagacg cgctggtgcc
    12361 gcgcacaccc agcccgcggc catcgacctc ggcggcggat ttggccattg cgcccaagaa
    12421 gaaaaagaag cgcccttctc ccaagcccga gcgcccgcca tcaccagagg taatcgtgga
    12481 cagcgaggaa gaaagagaag atgtggcgct acaaatggtg ggtttcagca acccaccggt
    12541 gctaatcaag catggcaaag gaggtaagcg cacagtgcgg cggctgaatg aagacgaccc
    12601 agtggcgcgt ggtatgcgga cgcaagagga agaggaagag cccagcgaag cggaaagtga
    12661 aattacggtg atgaacccgc tgagtgtgcc gatcgtgtct gcgtgggaga agggcatgga
    12721 ggctgcgcgc gcgctgatgg acaagtacca cgtggataac gatctaaagg cgaacttcaa
    12781 actactgcct gaccaagtgg aagctctggc ggccgtatgc aagacctggc tgaacgagga
    12841 gcaccgcggg ttgcagctga ccttcaccag caacaagacc tttgtgacga tgatggggcg
    12901 attcctgcag gcgtacctgc agtcgtttgc agaggtgacc tacaagcatc acgagcccac
    12961 gggctgcgcg ttgtggctgc accgctgcgc tgagatcgaa ggcgagctta agtgtctaca
    13021 cggaagcatt atgataaata aggagcacgt gattgaaatg gatgtgacga gcgaaaacgg
    13081 gcagcgcgcg ctgaaggagc agtctagcaa ggccaagatc gtgaagaacc ggtggggccg
    13141 aaatgtggtg cagatctcca acaccgacgc aaggtgctgc gtgcacgacg cggcctgtcc
    13201 ggccaatcag ttttccggca agtcttgcgg catgttcttc tctgaaggcg caaaggctca
    13261 ggtggctttt aagcagatca aggcttttat gcaggcgctg tatcctaacg cccagaccgg
    13321 gcacggtcac cttttgatgc cactacggtg cgagtgcaac tcaaagcctg ggcacgcgcc
    13381 ctttttggga aggcagctac caaagttgac tccgttcgcc ctgagcaacg cggaggacct
    13441 ggacgcggat ctgatctccg acaagagcgt gctggccagc gtgcaccacc cggcgctgat
    13501 agtgttccag tgctgcaacc ctgtgtatcg caactcgcgc gcgcagggcg gaggccccaa
    13561 ctgcgacttc aagatatcgg cgcccgacct gctaaacgcg ttggtgatgg tgcgcagcct
    13621 gtggagtgaa aacttcaccg agctgccgcg gatggttgtg cctgagttta agtggagcac
    13681 taaacaccag tatcgcaacg tgtccctgcc agtggcgcat agcgatgcgc ggcagaaccc
    13741 ctttgatttt taaacggcgc agacggcaag ggtgggggta aataatcacc cgagagtgta
    13801 caaataaaag catttgcctt tattgaaagt gtctctagta cattattttt acatgttttt
    13861 caagtgacaa aaagaagtgg cgctcctaat ctgcgcactg tggctgcgga agtagggcga
    13921 gtggcgctcc aggaagctgt agagctgttc ctggttgcga cgcagggtgg gctgtacctg
    13981 gggactgttg agcatggagt tgggtacccc ggtaataagg ttcatggtgg ggttgtgatc
    14041 catgggagtt tggggccagt tggcaaaggc gtggagaaac atgcagcaga atagtccaca
    14101 ggcggccgag ttgggcccct gtacgctttg ggtggacttt tccagcgtta tacagcggtc
    14161 gggggaagaa gcaatggcgc tacggcgcag gagtgactcg tactcaaact ggtaaacctg
    14221 cttgagtcgc tggtcagaaa agccaaaggg ctcaaagagg tagcatgttt ttgagtgcgg
    14281 gttccaggca aaggccatcc agtgtacgcc cccagtctcg gtccgagact cgaaccgggg
    14341 gtcccgcgac tcaacccttg gaaaataacc ctccggctac agggagcgag ccacttaatg
    14401 ctttcgcttt ccagcctaac cgcttacgct gcgcgcggcc agtggccaaa aaagctagcg
    14461 cagcagccgc cgcgcctgga aggaagccaa aaggagcact cccccgttgt ctgacgtcgc
    14521 acacctgggt tcgacacgcg ggcggtaacc gcatggatca cggcggacgg ccggatacgg
    14581 ggctcgaacc ccggtcgtcc gccatgatac ccttgcgaat ttatccacca gaccacggaa
    14641 gagtgcccgc ttacaggctc tccttttgca cgctagagcg tcaacgattg cgcgcgcctg
    14701 accggccaga gcgtcccgac catggagcac tttttgccgc tgcgcaacat ctggaaccgc
    14761 gtccgcgact ttccgcgcgc ctccaccacc gccgccggca tcacctggat gtccaggtac
    14821 atctacggat atcatcgcct tatgttggaa gatctcgccc ccggagcccc ggccacccta
    14881 cgctggcccc tctaccgcca gccgccgccg cactttttgg tgggatacca gtacctggtg
    14941 cggacttgca acgactacgt atttgactcg agggcttact cgcgtctcag gtacaccgag
    15001 ctctcgcagc cgggtcacca gaccgttaac tggtccgtta tggccaactg cacttacacc
    15061 atcaacacgg gcgcatacca ccgctttgtg gacatggatg acttccagtc taccctcacg
    15121 caggtgcagc aggccatatt agccgagcgc gttgtcgccg acctagccct gcttcagccg
    15181 atgaggggct tcggggtcac acgcatggga ggaagagggc gccacctacg gccaaactcc
    15241 gccgccgccg cagcgataga tgcaagagat gcaggacaag aggaaggaga agaagaagtg
    15301 ccggtagaaa ggctcatgca agactactac aaagacctgc gccgatgtca aaacgaagcc
    15361 tggggcatgg ccgaccgcct gcgcattcag caggccggac ccaaggacat ggtgcttctg
//

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